Anti-Maltose Binding Protein antibody [MBP-17] (HRP) (ab49923)

Overview

  • Product name
    Anti-Maltose Binding Protein antibody [MBP-17] (HRP)
    See all Maltose Binding Protein primary antibodies
  • Description
    Mouse monoclonal [MBP-17] to Maltose Binding Protein (HRP)
  • Conjugation
    HRP
  • Specificity
    This antibody recognizes native as well as denatured reduced forms of purified Maltose Binding Protein (MBP) or MBP fusion proteins.
  • Tested applications
    Suitable for: WB, ELISA, Dot blotmore details
  • Immunogen

    Recombinant full length Maltose Binding Protein fusion protein

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.0268% PBS, 1% BSA
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    The immunoglobulin fraction is purified from ascites fluid of the MBP-17 hybridoma and then conjugated with horseradish peroxidase (HRP).
  • Clonality
    Monoclonal
  • Clone number
    MBP-17
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab49923 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 43 kDa.
ELISA Use at an assay dependent dilution.
Dot blot Use at an assay dependent dilution.

Target

  • Relevance
    Epitope tagging offers an easy and universal strategy for the identification and purification of proteins derived by recombinant DNA technology. The insertion of a Maltose Binding Protein (MBP) tag creates a stable fusion product that does not interfere with the bioactivity of the protein or with the biodistribution of the MBP tagged product. Cleavage by factor Xa separates MBP from its partner protein.
  • Cellular localization
    Periplasm
  • Alternative names
    • ECK4026 antibody
    • JW3994 antibody
    • Mal E antibody
    • MalE antibody
    • malJ antibody
    • Maltodextrin binding protein antibody
    • Maltose ABC transporter periplasmic protein antibody
    • Maltose binding periplasmic protein antibody
    • MMBP antibody
    • Periplasmic maltose binding protein antibody
    see all

Anti-Maltose Binding Protein antibody [MBP-17] (HRP) images

  • ab49923 staining Maltose binding protein by ELISA. The sample was blocked in 1% milk for 1 hour at 18°C. The primary was diluted at 1/2000 in PBS-Tween20 buffer and incubated with sample for 1 hour at 18°C.

    See Abreview

References for Anti-Maltose Binding Protein antibody [MBP-17] (HRP) (ab49923)

This product has been referenced in:
  • Ishida K  et al. Immunolocalization of anti-hsf1 to the acetabular glands of infectious schistosomes suggests a non-transcriptional function for this transcriptional activator. PLoS Negl Trop Dis 8:e3051 (2014). Read more (PubMed: 25078989) »
  • Beck J  et al. Ubiquitylation-dependent localization of PLK1 in mitosis. Nat Cell Biol 15:430-9 (2013). Read more (PubMed: 23455478) »

See all 4 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
ELISA
Sample
Saccharomyces cerevisiae Recombinant protein (Recombinant Yeast SUMO protein)
Specification
Recombinant Yeast SUMO protein
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Type
Direct
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Abcam user community

Verified customer

Submitted May 12 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Escherichia coli Purified protein (BL21(DE3))
Loading amount
1 µg
Specification
BL21(DE3)
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: RT°C
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Abcam user community

Verified customer

Submitted Jul 22 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
ELISA
Sample
Rat Recombinant protein (neuronal)
Specification
neuronal
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 18°C
Type
Indirect
Username

Abcam user community

Verified customer

Submitted May 06 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Escherichia coli Purified protein (E.Coli)
Loading amount
0.05 µg
Specification
E.Coli
Gel Running Conditions
Reduced Denaturing (gradient 4-12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Jan 09 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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