Anti-Maltose Binding Protein antibody [MBP-17] (HRP) (ab49923)

Overview

  • Product nameAnti-Maltose Binding Protein antibody [MBP-17] (HRP)
    See all Maltose Binding Protein primary antibodies
  • Description
    Mouse monoclonal [MBP-17] to Maltose Binding Protein (HRP)
  • ConjugationHRP
  • SpecificityThis antibody recognizes native as well as denatured reduced forms of purified Maltose Binding Protein (MBP) or MBP fusion proteins.
  • Tested applicationsSuitable for: WB, ELISA, Dot blotmore details
  • Immunogen

    Recombinant full length Maltose Binding Protein fusion protein

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.01% Thimerosal (merthiolate)
    Constituents: 0.0268% PBS, 1% BSA
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesThe immunoglobulin fraction is purified from ascites fluid of the MBP-17 hybridoma and then conjugated with horseradish peroxidase (HRP).
  • ClonalityMonoclonal
  • Clone numberMBP-17
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab49923 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 43 kDa.
ELISA Use at an assay dependent dilution.
Dot blot Use at an assay dependent dilution.

Target

  • RelevanceEpitope tagging offers an easy and universal strategy for the identification and purification of proteins derived by recombinant DNA technology. The insertion of a Maltose Binding Protein (MBP) tag creates a stable fusion product that does not interfere with the bioactivity of the protein or with the biodistribution of the MBP tagged product. Cleavage by factor Xa separates MBP from its partner protein.
  • Cellular localizationPeriplasm
  • Alternative names
    • ECK4026 antibody
    • JW3994 antibody
    • Mal E antibody
    • MalE antibody
    • malJ antibody
    • Maltodextrin binding protein antibody
    • Maltose ABC transporter periplasmic protein antibody
    • Maltose binding periplasmic protein antibody
    • MMBP antibody
    • Periplasmic maltose binding protein antibody
    see all

Anti-Maltose Binding Protein antibody [MBP-17] (HRP) images

  • ab49923 staining Maltose binding protein by ELISA. The sample was blocked in 1% milk for 1 hour at 18°C. The primary was diluted at 1/2000 in PBS-Tween20 buffer and incubated with sample for 1 hour at 18°C.

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References for Anti-Maltose Binding Protein antibody [MBP-17] (HRP) (ab49923)

This product has been referenced in:
  • Ishida K  et al. Immunolocalization of anti-hsf1 to the acetabular glands of infectious schistosomes suggests a non-transcriptional function for this transcriptional activator. PLoS Negl Trop Dis 8:e3051 (2014). Read more (PubMed: 25078989) »
  • Beck J  et al. Ubiquitylation-dependent localization of PLK1 in mitosis. Nat Cell Biol 15:430-9 (2013). Read more (PubMed: 23455478) »

See all 4 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application ELISA
Sample Saccharomyces cerevisiae Recombinant protein (Recombinant Yeast SUMO protein)
Specification Recombinant Yeast SUMO protein
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Type Direct
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Abcam user community

Verified customer

Submitted May 12 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Escherichia coli Purified protein (BL21(DE3))
Loading amount 1 µg
Specification BL21(DE3)
Gel Running Conditions Reduced Denaturing (12%)
Blocking step Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: RT°C
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Verified customer

Submitted Jul 22 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application ELISA
Sample Rat Recombinant protein (neuronal)
Specification neuronal
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 18°C
Type Indirect
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Verified customer

Submitted May 06 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Escherichia coli Purified protein (E.Coli)
Loading amount 0.05 µg
Specification E.Coli
Gel Running Conditions Reduced Denaturing (gradient 4-12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Verified customer

Submitted Jan 09 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"