Anti-Maltose Binding Protein antibody [R29.6] (ab65)


  • Product nameAnti-Maltose Binding Protein antibody [R29.6]
    See all Maltose Binding Protein primary antibodies
  • Description
    Mouse monoclonal [R29.6] to Maltose Binding Protein
  • Tested applicationsSuitable for: ICC, IP, WB, ChIPmore details
  • Immunogen

    MOS maltose binding protein fusion protein.

  • General notesMBP is a bacterial protein, which is also used as a fusion protein in expression constructs.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • Clone numberR29.6
  • MyelomaSp2/0
  • IsotypeIgG1
  • Light chain typeunknown
  • Research areas


Our Abpromise guarantee covers the use of ab65 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration.
ChIP Use at an assay dependent dilution.


  • RelevanceEpitope tagging offers an easy and universal strategy for the identification and purification of proteins derived by recombinant DNA technology. The insertion of a Maltose Binding Protein (MBP) tag creates a stable fusion product that does not interfere with the bioactivity of the protein or with the biodistribution of the MBP tagged product. Cleavage by factor Xa separates MBP from its partner protein.
  • Cellular localizationPeriplasm
  • Alternative names
    • ECK4026 antibody
    • JW3994 antibody
    • Mal E antibody
    • MalE antibody
    • malJ antibody
    • Maltodextrin binding protein antibody
    • Maltose ABC transporter periplasmic protein antibody
    • Maltose binding periplasmic protein antibody
    • MMBP antibody
    • Periplasmic maltose binding protein antibody
    see all

Anti-Maltose Binding Protein antibody [R29.6] images

  • Immunofluorescence analysis of Saos-2 cells, transfected with full-length MBP-Apoptin and MBP-Apoptin(80–121). After fixation, the cells were stained for Maltose Binding Protein using ab65. Nuclear morphology is indicated by DAPI staining.
  • Image from Verhoeven GS et al., PLoS One. 2009 Aug 25;4(8):e6739.Fig S6.; doi:10.1371/journal.pone.0006739; August 25, 2009, PLoS ONE 4(8): e6739.

References for Anti-Maltose Binding Protein antibody [R29.6] (ab65)

This product has been referenced in:
  • Im YJ  et al. Structure and function of the ESCRT-II-III interface in multivesicular body biogenesis. Dev Cell 17:234-43 (2009). Read more (PubMed: 19686684) »
  • Verhoeven GS  et al. Differential bacterial surface display of peptides by the transmembrane domain of OmpA. PLoS One 4:e6739 (2009). WB . Read more (PubMed: 19707582) »

See all 5 Publications for this product

Product Wall

Unfortunately, the epitope recognized by product ab65 has not yet been mapped.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application ChIP
Sample Saccharomyces cerevisiae Cell (S. cerevisae)
Specification S. cerevisae
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 0 second(s)
Specification of the cross-linking agent: formaldehyde
Detection step Other
Positive control negative control: MBP-expressing vector in yeast

Mr. Xiao Liu

Verified customer

Submitted Aug 11 2005

This antibody was raised against a fusion protein and unfortunately we don't have the amino acid sequence.

I am afraid that this antibody has not been tested yet in ELISA. We also don't currently provide a conjugation service for our antibodies, however we do supply a range of conjugated secondary antibodies that may be suitable for your needs.

The optimal dilution should be determined for each application , but a good start point for Western blots would be 1µg/ml