Purification notesThis product is an IgG fraction antibody purified from monospecific antiserum by a multistep process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesDot: Use at an assay dependent dilution.
ELISA: 1/4000 - 1/20000. This product has been assayed against 1.0 µg of Maltose Phosphorylase in astandard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS as a substrate for 30 minutes at room temperature.
IM: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 86 kDa.
Suitable for antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceMaltose phosphorylase is a dimeric enzyme that catalyzes the conversion of maltose and inorganic phosphate into beta D glucose 1 phosphate and glucose without requiring any cofactors, such as pyridoxal phosphate. The enzyme is part of operons that are involved in maltose/malto oligosaccharide metabolism.
Maltose phosphorylase antibody
References for Anti-Maltose phosphorylase antibody (Biotin) (ab34543)
has not yet been referenced specifically in any publications.
Publishing research using ab34543? Please let us know so that we can cite the reference in this datasheet.
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