Anti-MAMDC2 antibody (ab121710)
Key features and details
- Rabbit polyclonal to MAMDC2
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MAMDC2 antibody
See all MAMDC2 primary antibodies -
Description
Rabbit polyclonal to MAMDC2 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
antigen sequence, corresponding to amino acids 322-419 (FSPVHCQNQT ELLFSAVEAS CNFEQDLCNF YQDKEGPGWT RVKVKPNMYR AGDHTTGLGY YLLANTKFTS QPGYIGRLYG PSLPGNLQYC LRFHYAIY) of Human MAMDC2.
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Positive control
- IHC: Human cervix, skin and gastrointestinal tissues. WB: HEK293T cell lysate ICC/IF: Human cell line U-2 OS shows
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab121710 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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IHC-P |
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
IHC-P
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Sequence similarities
Contains 4 MAM domains. -
Post-translational
modificationsO-glycosylated. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 256691 Human
- Omim: 612879 Human
- SwissProt: Q7Z304 Human
- Unigene: 547172 Human
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Alternative names
- MAM domain-containing protein 2 antibody
- MAM domain-containing proteoglycan antibody
- MAMC2_HUMAN antibody
see all
Images
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Immunofluorescent staining of human cell line U-2 OS shows localization to nuclear speckles & plasma membrane.
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Lanes 2-3 : Anti-MAMDC2 antibody (ab121710)
Lane 1 : Marker
Lane 2 : HEK293T cell lysate - vector only transfected
Lane 3 : HEK293T cell lysate - overexpressing MAMDC2 with a C-terminal myc-DDK tag (~3.1kDa) -
Immunohistochemical analysis of human cervix tissue labeling MAMDC2 with ab121710 at a 1/50 dilution.
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Immunohistochemical analysis of human gastrointestinal tissue labeling MAMDC2 with ab121710 at a 1/50 dilution.
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Immunohistochemical analysis of human skin tissue labeling MAMDC2 with ab121710 at a 1/50 dilution.
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Immunohistochemical analysis of human pancreas tissue labeling MAMDC2 with ab121710 at a 1/50 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab121710 has not yet been referenced specifically in any publications.