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Goat polyclonal to MAML1
Predicted to work with:
, corresponding to N terminal amino acids 2-15 of Human MAML1(Peptide available as
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline. pH 7.3
Concentration information loading...
Immunogen affinity purified
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunizing Peptide (Blocking)
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
Peptide Antibody detection limit dilution 1/128000.
Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 108 kDa.Can be blocked with
Human MAML1 peptide (ab23302).
Use a concentration of 2.5 - 3.8 mg/ml.
Acts as a transcriptional coactivator for NOTCH proteins. Has been shown to amplify NOTCH-induced transcription of HES1. Enhances phosphorylation and proteolytic turnover of the NOTCH intracellular domain in the nucleus through interaction with CDK8. Binds to CREBBP/CBP which promotes nucleosome acetylation at NOTCH enhancers and activates transcription. Induces phosphorylation and localization of CREBBP to nuclear foci. Plays a role in hematopoietic development by regulating NOTCH-mediated lymphoid cell fate decisions.
Widely expressed with highest levels in heart, pancreas, peripheral blood leukocytes and spleen.
Belongs to the mastermind family.
The C-terminal region is required for transcriptional activation.
Nucleus speckle. Nuclear, in a punctate manner.
Information by UniProt
MAML 1 antibody
Western blot - Anti-MAML1 antibody (ab17019)
Anti-MAML1 antibody (ab17019) at 0.1 µg/ml + Tissue lysates prepared from Human skeletal muscles at 35 µg
Predicted band size: 108 kDa
Immunohistochemistry (Paraffin-embedded sections) - Anti-MAML1 antibody (ab17019)
ab17019 (3.8µg/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"