Overview

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline. pH 7.3
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab17019 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

Peptide Antibody detection limit dilution 1/128000.

WB Use a concentration of 0.1 - 0.3 µg/ml. Predicted molecular weight: 108 kDa.Can be blocked with Human MAML1 peptide (ab23302).
IHC-P Use a concentration of 2.5 - 3.8 mg/ml.

Target

  • FunctionActs as a transcriptional coactivator for NOTCH proteins. Has been shown to amplify NOTCH-induced transcription of HES1. Enhances phosphorylation and proteolytic turnover of the NOTCH intracellular domain in the nucleus through interaction with CDK8. Binds to CREBBP/CBP which promotes nucleosome acetylation at NOTCH enhancers and activates transcription. Induces phosphorylation and localization of CREBBP to nuclear foci. Plays a role in hematopoietic development by regulating NOTCH-mediated lymphoid cell fate decisions.
  • Tissue specificityWidely expressed with highest levels in heart, pancreas, peripheral blood leukocytes and spleen.
  • Sequence similaritiesBelongs to the mastermind family.
  • DomainThe C-terminal region is required for transcriptional activation.
  • Cellular localizationNucleus speckle. Nuclear, in a punctate manner.
  • Information by UniProt
  • Database links
  • Alternative names
    • Mam-1 antibody
    • Mam1 antibody
    • MAML 1 antibody
    • Maml1 antibody
    • MAML1_HUMAN antibody
    • Mastermind like 1 antibody
    • Mastermind-like protein 1 antibody
    see all

Anti-MAML1 antibody images

  • Anti-MAML1 antibody (ab17019) at 0.1 µg/ml + Tissue lysates prepared from Human skeletal muscles at 35 µg

    Predicted band size : 108 kDa
  • ab17019 (3.8µg/ml) staining of paraffin embedded Human Placenta. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

References for Anti-MAML1 antibody (ab17019)

ab17019 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"