Overview

  • Product nameAnti-MAML1 antibody
    See all MAML1 primary antibodies
  • Description
    Rabbit polyclonal to MAML1
  • Tested applicationsSuitable for: IP, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse, Horse
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 950 to the C-terminus MAML1.

    (Peptide available as ab66491.)

  • Positive control
    • This antibody gave a positive signal in the following Lysates; SW480, HepG2, HeLa and Rat Thymus Tissue Lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab65090 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 117 kDa (predicted molecular weight: 108 kDa).
ICC/IF Use a concentration of 1 µg/ml.

Target

  • FunctionActs as a transcriptional coactivator for NOTCH proteins. Has been shown to amplify NOTCH-induced transcription of HES1. Enhances phosphorylation and proteolytic turnover of the NOTCH intracellular domain in the nucleus through interaction with CDK8. Binds to CREBBP/CBP which promotes nucleosome acetylation at NOTCH enhancers and activates transcription. Induces phosphorylation and localization of CREBBP to nuclear foci. Plays a role in hematopoietic development by regulating NOTCH-mediated lymphoid cell fate decisions.
  • Tissue specificityWidely expressed with highest levels in heart, pancreas, peripheral blood leukocytes and spleen.
  • Sequence similaritiesBelongs to the mastermind family.
  • DomainThe C-terminal region is required for transcriptional activation.
  • Cellular localizationNucleus speckle. Nuclear, in a punctate manner.
  • Information by UniProt
  • Database links
  • Alternative names
    • Mam-1 antibody
    • Mam1 antibody
    • MAML 1 antibody
    • Maml1 antibody
    • MAML1_HUMAN antibody
    • Mastermind like 1 antibody
    • Mastermind-like protein 1 antibody
    see all

Anti-MAML1 antibody images

  • MAML1 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to MAML1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

    The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab65090.

    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).

    Band: 117kDa; MAML1

  • All lanes : Anti-MAML1 antibody (ab65090) at 1 µg/ml

    Lane 1 : SW480 whole cell lysate (ab3957)
    Lane 2 : Rat Thymus Tissue Lysate
    Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 108 kDa
    Observed band size : 117 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 75 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab65090 stained HeLa cells. The cells were fixed with 4% PFA (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65090 at 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive result in 4% PFA fixed (10 min) MCF7 cells at 1µg/ml. This antibody also gave a positive result in 4% PFA fixed (10 min) MCF7 cells at 1µg/ml.

References for Anti-MAML1 antibody (ab65090)

ab65090 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab65090.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"