Overview

  • Product name
  • Description
    Rabbit polyclonal to MAML1
  • Specificity
    We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • Tested applications
    Suitable for: IHC-P, WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee
  • Immunogen

    Synthetic peptide, corresponding to a region between amino acids 75-125 of Human MAML1

  • Positive control
    • Whole cell lysate from HeLa cells

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7 to 8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab70488 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/1000 - 1/10000. Predicted molecular weight: 108 kDa.
IP Use at 1-4 µg/mg of lysate.

Target

  • Function
    Acts as a transcriptional coactivator for NOTCH proteins. Has been shown to amplify NOTCH-induced transcription of HES1. Enhances phosphorylation and proteolytic turnover of the NOTCH intracellular domain in the nucleus through interaction with CDK8. Binds to CREBBP/CBP which promotes nucleosome acetylation at NOTCH enhancers and activates transcription. Induces phosphorylation and localization of CREBBP to nuclear foci. Plays a role in hematopoietic development by regulating NOTCH-mediated lymphoid cell fate decisions.
  • Tissue specificity
    Widely expressed with highest levels in heart, pancreas, peripheral blood leukocytes and spleen.
  • Sequence similarities
    Belongs to the mastermind family.
  • Domain
    The C-terminal region is required for transcriptional activation.
  • Cellular localization
    Nucleus speckle. Nuclear, in a punctate manner.
  • Information by UniProt
  • Database links
  • Alternative names
    • Mam-1 antibody
    • Mam1 antibody
    • MAML 1 antibody
    • Maml1 antibody
    • MAML1_HUMAN antibody
    • Mastermind like 1 antibody
    • Mastermind-like protein 1 antibody
    see all

Anti-MAML1 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling MAML1 with ab70488 at 1/1000 (1µg/ml). Detection: DAB.
  • Anti-MAML1 antibody (ab70488) at 1 µg/ml + Whole cell lysate from HeLa cells at 50 µg
    Developed using the ECL technique

    Predicted band size : 108 kDa
    Observed band size : 120 kDa (why is the actual band size different from the predicted?)


    Exposure time : 30 seconds
  • Detection of Human MAML1 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab70488 at 3 µg/mg lysate for IP.
    Chemiluminescence with exposure times of 30 seconds.

References for Anti-MAML1 antibody (ab70488)

ab70488 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab70488.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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