Anti-Mammaglobin A antibody [31A5] (ab82203)

Overview

  • Product nameAnti-Mammaglobin A antibody [31A5]
    See all Mammaglobin A primary antibodies
  • Description
    Rabbit monoclonal [31A5] to Mammaglobin A
  • SpecificityReacts with human mammaglobin A, no cross-reaction with lypophilin B and lypophilin C (mammaglobin B).
  • Tested applicationsSuitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human, Monkey
  • Immunogen

    Recombinant full length protein (Human) Mammaglobin A protein

  • Positive control
    • Breast carcinoma, skin (eccrine sweat glands).

Properties

Applications

Our Abpromise guarantee covers the use of ab82203 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
IHC-P
  • Application notesELISA: Use at an assay dependent dilution.

    IHC-P: Use at a concentration of 1 µg/ml. Perform antigen retrieval step using Citrate buffer, or buffered EDTA, or Glycol based solution before commencing with IHC staining protocol.
    This antibody is suitable for the detection of occult mamma carcinoma cells in sentinel lymph nodes and in peripheral blood. The overall sensitivity of mammaglobin detection on paraffin sections of mamma carcinoma was reported to be >80% (Fanger et al. 2002; Han et al. 2003).

    WB: Use at an assay dependent dilution. See PubMed ID: 14521461

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    Anti-Mammaglobin A antibody [31A5] images

    • ab82203 at 1µg/ml staining Mammaglobin A in sentinel lymph nodes in metastasis of breast carcinoma.
    • ab82203 at 1µg/ml staining Mammaglobin A over-expression in breast carcinoma in situ.

    References for Anti-Mammaglobin A antibody [31A5] (ab82203)

    This product has been referenced in:
    • Plante I  et al. Cx43 suppresses mammary tumor metastasis to the lung in a Cx43 mutant mouse model of human disease. Oncogene : (2010). IHC-P ; Mouse . Read more (PubMed: 21151177) »
    • Han JH  et al. Mammaglobin expression in lymph nodes is an important marker of metastatic breast carcinoma. Arch Pathol Lab Med 127:1330-4 (2003). WB, IHC-P, ELISA ; Human, Mouse, Monkey . Read more (PubMed: 14521461) »

    See all 3 Publications for this product

    Product Wall

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (4T1 breast tumor metastases in lung and liver)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate
    Permeabilization Yes - Triton
    Specification 4T1 breast tumor metastases in lung and liver
    Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
    Fixative Formaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted May 27 2015

    Thank you for getting back to me and for confirming the order details. Currently, ab82203 is the only antibody against Mammaglobin A which has been tested for cross-reaction with mouse samples. Although, we have some other antibodies against the same t...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"