Overview

  • Product nameAnti-Mammaglobin A antibody
    See all Mammaglobin A primary antibodies
  • Description
    Rabbit polyclonal to Mammaglobin A
  • SpecificityNo cross-reactivity to lipophilin A, lipophilin B (BU101), lipophilin C (lacryglobin, mammaglobin B), or uteroglobin (Clara cell protein, Clara cell 10 kDa protein) by Western blot analysis of bacterially produced recombinant proteins.
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide: EVFMQLIYDSSLCDLF conjugated to KLH, corresponding to C terminal amino acids 78 - 93 of Human mammaglobin.

  • Positive control
    • Human breast cancer cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab18517 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 15 kDa.
IHC-P Use at an assay dependent concentration.

Target

  • Tissue specificityMammary gland specific. Over-expressed in breast cancer.
  • Sequence similaritiesBelongs to the secretoglobin family. Lipophilin subfamily.
  • Information by UniProt
  • Database links
  • Alternative names
    • Mammaglobin 1 antibody
    • Mammaglobin-1 antibody
    • Mammaglobin-A antibody
    • MGB1 antibody
    • Scgb2a2 antibody
    • Secretoglobin family 2A member 2 antibody
    • SG2A2 antibody
    • SG2A2_HUMAN antibody
    • UGB2 antibody
    see all

Anti-Mammaglobin A antibody images

  • IHC image of ab18517 staining in human breast carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab18517, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-Mammaglobin A antibody (ab18517)

This product has been referenced in:
  • Long W  et al. SRC-3Delta4 mediates the interaction of EGFR with FAK to promote cell migration. Mol Cell 37:321-32 (2010). IHC-P ; Mouse . Read more (PubMed: 20159552) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"