Mammalian Cell Lysis Buffer 5X (ab179835)
Key features and details
- Sample type: Adherent cells, Suspension cells, Tissue
Overview
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Product name
Mammalian Cell Lysis Buffer 5X -
Sample type
Tissue, Adherent cells, Suspension cells -
Product overview
Mammalian Cell Lysis Buffer 5X (ab179835) is widely used to prepare mammalian cell and tissue lysates for use in a variety of downstream biochemical assays, especially those for quantification of enzymatic activity. This buffer does not contain SDS, a reagent that interferes with many activity assays.
The lysis buffer has been optimized for mammalian samples, but it can also be used for preparation of plant or bacterial samples.
The buffer just requires a simple 5-fold dilution, and minimal hands-on-time. Cell lysates are ready in only 15 minutes.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 10 ml Mammalian Cell lysis buffer 5X 1 x 10ml -
Research areas
Datasheets and documents
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SDS download
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Datasheet download
References (17)
ab179835 has been referenced in 17 publications.
- Upadhyay PR et al. Differential Induction of Reactive Oxygen Species and Expression of Antioxidant Enzymes in Human Melanocytes Correlate with Melanin Content: Implications on the Response to Solar UV and Melanoma Susceptibility. Antioxidants (Basel) 11:N/A (2022). PubMed: 35740103
- Ji Y et al. The Acid Sphingomyelinase Inhibitor Amitriptyline Ameliorates TNF-a-Induced Endothelial Dysfunction. Cardiovasc Drugs Ther N/A:N/A (2022). PubMed: 36103099
- Lu F et al. Thioredoxin 1 supports colorectal cancer cell survival and promotes migration and invasion under glucose deprivation through interaction with G6PD. Int J Biol Sci 18:5539-5553 (2022). PubMed: 36147458
- Barradas M et al. Fatty acids homeostasis during fasting predicts protection from chemotherapy toxicity. Nat Commun 13:5677 (2022). PubMed: 36167809
- Zhang P et al. Pannexin 3 regulates skin development via Epiprofin. Sci Rep 11:1779 (2021). PubMed: 33469169