The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 73 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
RelevanceMAN1A2 plays a role in the maturation of Asn-linked oligosaccharides (N-glycans) in mammalian cells, by progressively excising alpha-1,2-linked mannose residues from Man(9)GlcNAc(2) to produce Man(5)GlcNAc(2).
Cellular localizationGolgi apparatus membrane; Single-pass type II membrane protein