The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/10. Predicted molecular weight: 26 kDa. On a 12% non-reducing SDS-PAGE a prominent band of ~50Kda is detected. Under reducing conditions a 26 KDa band is detected.
Use at an assay dependent dilution. Biotinylated ab106046 was used as detector in ELISA at 1µg/ml TBS/Tween-20/Ca.
1/10. Cryostat sections (7µm) of mouse tissue were acetone fixed and stained o/n at 4C in TBS/2% goat serum
Use at an assay dependent dilution. Hyphae of C. albicans were fixed on poly-l-lysine coated slides with ice-cold acetone. Slides were incubated for 1h at RT with ab106046.
Calcium-dependent lectin involved in innate immune defense. Binds mannose, fucose and N-acetylglucosamine on different microorganisms and activates the lectin complement pathway. Binds to late apoptotic cells, as well as to apoptotic blebs and to necrotic cells, but not to early apoptotic cells, facilitating their uptake by macrophages. May bind DNA.
Plasma protein produced mainly in the liver.
Involvement in disease
Note=There is an association between low levels of MBL2 and a defect of opsonization which results in susceptibility to frequent and chronic infections.
ab106046 detecting Mannan Binding Lectin deposition in developing murine atherosclerotic lesions following 10 weeks of high fat feeding. Mannan Binding Lectin was detected in and around invading macrophages invading the intima (insert). Mannan Binding Lectin bound at sites of necrosis (upper right corner). No Mannan Binding Lectin binding was shown in the media or on fibrous caps covering the thickened intima.
Garland DL et al. Mouse genetics and proteomic analyses demonstrate a critical role for complement in a model of DHRD/ML, an inherited macular degeneration. Hum Mol GenetN/A:N/A (2013).
Read more (PubMed: 23943789) »
Matthijsen RA et al. Macrophage-specific expression of mannose-binding lectin controls atherosclerosis in low-density lipoprotein receptor-deficient mice. Circulation119:2188-95 (2009).
Read more (PubMed: 19380618) »