Anti-Mannan Binding Lectin/MBL antibody [1E2] (ab23458)
Key features and details
- Mouse monoclonal [1E2] to Mannan Binding Lectin/MBL
- Suitable for: ELISA, WB
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Mannan Binding Lectin/MBL antibody [1E2]
See all Mannan Binding Lectin/MBL primary antibodies -
Description
Mouse monoclonal [1E2] to Mannan Binding Lectin/MBL -
Host species
Mouse -
Tested applications
Suitable for: ELISA, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length protein corresponding to Human Mannan Binding Lectin/MBL.
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General notes
ab23458 reacts with human MBL both in its poltmeric conforation and as a single subunit of moleculat mass 26 kDa. Can be applied for quantitative measurement of MBL in plasma or serum.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituents: 0.0268% PBS, 2.9% Sodium chloride -
Concentration information loading...
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Purity
Protein G purified -
Primary antibody notes
Ab 23458 reacts with human MBL both in its poltmeric conforation and as a single subunit of moleculat mass 26 kDa. Can be applied for quantitative measurement of MBL in plasma or serum. -
Clonality
Monoclonal -
Clone number
1E2 -
Myeloma
x63-Ag8.653 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab23458 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
1/10000.
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WB |
Use at an assay dependent dilution. Predicted molecular weight: 26 kDa.
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Notes |
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ELISA
1/10000. |
WB
Use at an assay dependent dilution. Predicted molecular weight: 26 kDa. |
Target
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Function
Calcium-dependent lectin involved in innate immune defense. Binds mannose, fucose and N-acetylglucosamine on different microorganisms and activates the lectin complement pathway. Binds to late apoptotic cells, as well as to apoptotic blebs and to necrotic cells, but not to early apoptotic cells, facilitating their uptake by macrophages. May bind DNA. -
Tissue specificity
Plasma protein produced mainly in the liver. -
Involvement in disease
Note=There is an association between low levels of MBL2 and a defect of opsonization which results in susceptibility to frequent and chronic infections. -
Sequence similarities
Contains 1 C-type lectin domain.
Contains 1 collagen-like domain. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 4153 Human
- Omim: 154545 Human
- SwissProt: P11226 Human
- Unigene: 499674 Human
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Alternative names
- COLEC 1 antibody
- COLEC1 antibody
- Collectin-1 antibody
see all
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab23458 has been referenced in 4 publications.
- Liu C et al. Exosomes Derived from miR-146a-5p-Enriched Mesenchymal Stem Cells Protect the Cardiomyocytes and Myocardial Tissues in the Polymicrobial Sepsis through Regulating MYBL1. Stem Cells Int 2021:1530445 (2021). PubMed: 34691188
- Harboe M et al. Design of a complement mannose-binding lectin pathway-specific activation system applicable at low serum dilutions. Clin Exp Immunol 144:512-20 (2006). PubMed: 16734621
- Downing I et al. Immature dendritic cells possess a sugar-sensitive receptor for human mannan-binding lectin. Immunology 109:360-4 (2003). PubMed: 12807481
- Sund S et al. Complement activation in early protocol kidney graft biopsies after living-donor transplantation. Transplantation 75:1204-13 (2003). PubMed: 12717204