Anti-Mannan Binding Lectin antibody [3B6] (ab23457)

Overview

  • Product name
    Anti-Mannan Binding Lectin antibody [3B6]
    See all Mannan Binding Lectin primary antibodies
  • Description
    Mouse monoclonal [3B6] to Mannan Binding Lectin
  • Specificity
    ab23457 is specific for MBL from human serum or plasma. In Western blotting, ab121075 reacts with Human MBL in both its oligomerized state.
  • Tested applications
    Suitable for: WB, IHC-Fr, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length native protein (purified) corresponding to Human Mannan Binding Lectin. Human MBL purified from Human donor plasma.

  • Epitope
    The epitope is on the head-neck region of the MBL protein chain.
  • Positive control
    • Human liver, Human cancerous liver and Human brain tissues.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 15mM Sodium Azide
    Constituents: 0.5M Sodium chloride, 0.01M PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    3B6
  • Myeloma
    x63-Ag8.653
  • Isotype
    IgG1
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab23457 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 26 kDa.
IHC-Fr Use at an assay dependent concentration.
IHC-P 1/250.
ELISA 1/16000.

Target

  • Function
    Calcium-dependent lectin involved in innate immune defense. Binds mannose, fucose and N-acetylglucosamine on different microorganisms and activates the lectin complement pathway. Binds to late apoptotic cells, as well as to apoptotic blebs and to necrotic cells, but not to early apoptotic cells, facilitating their uptake by macrophages. May bind DNA.
  • Tissue specificity
    Plasma protein produced mainly in the liver.
  • Involvement in disease
    Note=There is an association between low levels of MBL2 and a defect of opsonization which results in susceptibility to frequent and chronic infections.
  • Sequence similarities
    Contains 1 C-type lectin domain.
    Contains 1 collagen-like domain.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • COLEC 1 antibody
    • COLEC1 antibody
    • Collectin-1 antibody
    • HSMBPC antibody
    • Lectin, mannose-binding, soluble, 2 antibody
    • Mannan binding lectin antibody
    • Mannan binding protein antibody
    • Mannan-binding protein antibody
    • Mannose binding lectin (protein C) 2 soluble antibody
    • Mannose binding lectin (protein C) 2, soluble (opsonic defect) antibody
    • Mannose binding lectin (protein C) 2, soluble antibody
    • Mannose binding lectin 2 soluble antibody
    • Mannose binding lectin 2, soluble (opsonic defect) antibody
    • Mannose binding lectin antibody
    • Mannose binding lectin protein C2 soluble opsonic defect antibody
    • Mannose binding protein antibody
    • Mannose binding protein C antibody
    • Mannose binding protein C precursor antibody
    • Mannose binding protein, serum antibody
    • Mannose-binding lectin antibody
    • Mannose-binding protein C antibody
    • MBL 2 antibody
    • MBL antibody
    • MBL2 antibody
    • MBL2_HUMAN antibody
    • MBL2D antibody
    • MBP 1 antibody
    • MBP antibody
    • MBP C antibody
    • MBP-C antibody
    • MBP1 antibody
    • MBPB antibody
    • MBPC antibody
    • MBPD antibody
    • MGC116832 antibody
    • MGC116833 antibody
    • Opsonic defect antibody
    • protein C antibody
    • Soluble mannose binding lectin antibody
    see all

Anti-Mannan Binding Lectin antibody [3B6] images

  • ab23457 at 1 mg/ml staining human liver by IHC-P
  • ab23457 staining MBL in Paraffin-embedded Human Liver tissue by Immunohistochemistry.

  • ab23457 staining MBL in Paraffin-embedded Human cancerous liver tissue by Immunohistochemistry.

References for Anti-Mannan Binding Lectin antibody [3B6] (ab23457)

This product has been referenced in:
  • Yadav AK  et al. Differential expression of collectins in human placenta and role in inflammation during spontaneous labor. PLoS One 9:e108815 (2014). WB ; Human . Read more (PubMed: 25303045) »
  • Debiec H  et al. Recurrent membranous nephropathy in an allograft caused by IgG3? targeting the PLA2 receptor. J Am Soc Nephrol 23:1949-54 (2012). Read more (PubMed: 23123401) »

See all 13 Publications for this product

Product Wall

Thank you for this additional information. A few suggestions just to follow a protocol a little more similar to how we have tested. We generally recommend blocking in 5% milk in TBST and incubating the primary antibody overnight at 4 degrees. You may a...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (brain tissue)
Specification
brain tissue
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5%
Username

Vlady Pavlova

Verified customer

Submitted Aug 15 2006

Thank you for your enquiry. The antibody has been tested in IHC in human liver and brain sections, but we do not have those images unfortunately. The reference Holmskov UL Collectins and collectin receptors in innate immunity. APMIS Suppl 100:1-59 (20...

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Thank you for your enquiry. I have been informed by our lab that the antiserum was prepared in 0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15mM sodium azide. Please do not hesitate to contact me should you require further assistance.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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