Anti-Mannose Phosphate Isomerase antibody (ab95972)

Overview

  • Product name
    Anti-Mannose Phosphate Isomerase antibody
    See all Mannose Phosphate Isomerase primary antibodies
  • Description
    Rabbit polyclonal to Mannose Phosphate Isomerase
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant fragment, corresponding to amino acids 150-409 of Human Mannose Phosphate Isomerase (NP_002426)

  • Positive control
    • 293T, A431, H1299, HeLaS3, HepG2, MOLT4 and Raji lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab95972 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 46 kDa.
IHC-P 1/100 - 1/250.

Target

  • Function
    Involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions.
  • Tissue specificity
    Expressed in all tissues, but more abundant in heart, brain and skeletal muscle.
  • Pathway
    Nucleotide-sugar biosynthesis; GDP-alpha-D-mannose biosynthesis; alpha-D-mannose 1-phosphate from D-fructose 6-phosphate: step 1/2.
  • Involvement in disease
    Defects in MPI are the cause of congenital disorder of glycosylation type 1B (CDG1B) [MIM:602579]; also known as carbohydrate-deficient glycoprotein syndrome type Ib (CDGS1B). Congenital disorders of glycosylation are metabolic deficiencies in glycoprotein biosynthesis that usually cause severe mental and psychomotor retardation. They are characterized by under-glycosylated serum glycoproteins. CDG1B is clinically characterized by protein-losing enteropathy.
  • Sequence similarities
    Belongs to the mannose-6-phosphate isomerase type 1 family.
  • Cellular localization
    Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • PMI1 antibody
    • CDG1B antibody
    • FLJ39201 antibody
    • Mannose 6 phosphate isomerase antibody
    • Mannose-6-phosphate isomerase antibody
    • MANNOSEPHOSPHATE ISOMERASE antibody
    • MGC94106 antibody
    • MPI antibody
    • MPI_HUMAN antibody
    • Phosphohexomutase antibody
    • phosphomannose isomerase 1 antibody
    • Phosphomannose isomerase antibody
    • PMI antibody
    see all

Images

  • All lanes : Anti-Mannose Phosphate Isomerase antibody (ab95972) at 1/1000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : A431 whole cell lysate
    Lane 3 : H1299 whole cell lysate
    Lane 4 : HeLa S3 whole cell lysate
    Lane 5 : HepG2 whole cell lysate
    Lane 6 : MOLT4 whole cell lysate
    Lane 7 : Raji whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 46 kDa
  • Immunohistochemical analysis of paraffin-embedded human gastric CAN87 using ab95972 at 1/100 dilution.

References

ab95972 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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