Anti-Mannose Receptor antibody [15-2] (Biotin) (ab8919)

Overview

  • Product nameAnti-Mannose Receptor antibody [15-2] (Biotin)
    See all Mannose Receptor primary antibodies
  • Description
    Mouse monoclonal [15-2] to Mannose Receptor (Biotin)
  • ConjugationBiotin
  • Tested applicationsSuitable for: IHC-Fr, Flow Cyt, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length native protein from placental tissue (purified) (Human)

  • General notes


    The Mannose Receptor (MR), a member of the vertebrate C-type lectin family, is a pattern recognition receptor that is involved in both innate and adaptive immunity. The 180 kDa transmembrane protein consists of 5 domains: an amino-terminal cysteine-rich region, a fibronectin type II repeat, a series of eight tandem lectin-like carbohydrate recognition domains (responsible for the recognition of mannose and fucose), a transmembrane domain, and an intracellular carboxy-terminal tail. The structure is shared by the family of multi lectin mannose receptors: the phospholipase A2-receptor, DEC 205 and the novel C-type lectin receptor (mannose receptor X). The MR recognises a wide range of gram positive and gram negative bacteria, yeasts, parasites and mycobacteria. The MR has also been shown to bind and internalize tissue-type plasminogen activator. MR's are present on monocytes and dendritic cells (DC) and are presumed to play a role in innate and adaptive immunity, the latter via processing by DC. The expression of MR as observed in immunohistology is present on tissue macrophages, dendritic cells, a subpopulation of endothelial cells, Kupffer cells and sperm cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPBS , 0.1% BSA and 0.02% sodium azide
  • Concentration information loading...
  • PurityIgG fraction
  • Primary antibody notesThe Mannose Receptor (MR), a member of the vertebrate C-type lectin family, is a pattern recognition receptor that is involved in both innate and adaptive immunity. The 180 kDa transmembrane protein consists of 5 domains: an amino-terminal cysteine-rich region, a fibronectin type II repeat, a series of eight tandem lectin-like carbohydrate recognition domains (responsible for the recognition of mannose and fucose), a transmembrane domain, and an intracellular carboxy-terminal tail. The structure is shared by the family of multi lectin mannose receptors: the phospholipase A2-receptor, DEC 205 and the novel C-type lectin receptor (mannose receptor X). The MR recognises a wide range of gram positive and gram negative bacteria, yeasts, parasites and mycobacteria. The MR has also been shown to bind and internalize tissue-type plasminogen activator. MR's are present on monocytes and dendritic cells (DC) and are presumed to play a role in innate and adaptive immunity, the latter via processing by DC. The expression of MR as observed in immunohistology is present on tissue macrophages, dendritic cells, a subpopulation of endothelial cells, Kupffer cells and sperm cells.
  • ClonalityMonoclonal
  • Clone number15-2
  • Myelomaunknown
  • IsotypeIgG1
  • Light chain typeunknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab8919 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. ab18434-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB Use at an assay dependent concentration.

Target

  • FunctionMediates the endocytosis of glycoproteins by macrophages. Binds both sulfated and non-sulfated polysaccharide chains. Acts as phagocytic receptor for bacteria, fungi and other pathogens.
  • Sequence similaritiesContains 8 C-type lectin domains.
    Contains 1 fibronectin type-II domain.
    Contains 1 ricin B-type lectin domain.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • bA541I19.1 antibody
    • C type lectin domain family 13 member D antibody
    • C-type lectin domain family 13 member D antibody
    • CD 206 antibody
    • CD206 antibody
    • CD206 antigen antibody
    • CLEC13D antibody
    • CLEC13DL antibody
    • Macrophage mannose receptor 1 antibody
    • Macrophage mannose receptor 1 like protein 1 antibody
    • Macrophage mannose receptor antibody
    • Mannose receptor C type 1 antibody
    • Mannose receptor C type 1 like 1 antibody
    • MMR antibody
    • MRC 1 antibody
    • MRC1 antibody
    • MRC1_HUMAN antibody
    • MRC1L1 antibody
    • OTTHUMP00000045206 antibody
    see all

References for Anti-Mannose Receptor antibody [15-2] (Biotin) (ab8919)

This product has been referenced in:
  • Cominelli A  et al. Matrix metalloproteinase-27 is expressed in CD163+/CD206+ M2 macrophages in the cycling human endometrium and in superficial endometriotic lesions. Mol Hum Reprod 20:767-75 (2014). IHC-Fr ; Human . Read more (PubMed: 24810263) »
  • Zeyda M  et al. Osteopontin is an activator of human adipose tissue macrophages and directly affects adipocyte function. Endocrinology 152:2219-27 (2011). Read more (PubMed: 21467192) »

See all 6 Publications for this product

Product Wall

Thank you for contacting us.

Both of these product are tested and guaranteed to work in Flow Cytometry. These are specific against extracellular domain so the permeabilization step is optional; the antibodies would be able to stain the cells w...

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Thank you for your reply.


I would recommend ab6787 as a suitable secondary antibody to use with ab8919. Below please find the discount information for testing ab8919 in IHC-P.


I am very pleased to hear you would like to acce...

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Thank you for your reply.


I would like to recommend the following:


Primary antibody: ab6640 (F4/80 antibody)
Secondary antibody: ab99671 (Mouse monoclonal to Rat IgG2b - FITC)


compatible with


...

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Thank you for contacting Abcam and for your interest in our products.


In order to assist you further, I would like to clarify the details of your experiment. You mention that you are going to be performing IHC - will this be on frozen or ...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused...

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Thank you for your enquiry. The relevance section of this antibody was generated a while ago. Unfortunately I do not have information as to where the information "The expression of MR on monocytes increases during culture and can be enhanced by cyto...

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Thank you for your enquiry. Yes, you are right. The only difference between ab8919 and ab8918 is that ab8919 is Biotin conjugated. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

Thank you for your enquiry. Each antibody and batch of conjugate should be titrated to determine the optimal working dilutions. We would suggest that you should carry out a preliminary experiment to test different dilutions of the primary antibo...

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Thank you for your enquiry. Each antibody and batch of conjugate should be titrated to determine the optimal working dilutions. We would suggest that you should carry out a preliminary experiment to test different dilutions of the primary antibo...

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The antibody has been labeled with Biotin-X-NHS (Calbiochem-Novabiochem; cat# 203188) During the biotin labeling the free amino groups of the antibody react with biotin-N-hydroxy-succinimide ester (biotin-NHS).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"