Anti-Mannose Receptor antibody [EPR6828(B)] (ab125028)

Overview

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD) KD = 1.75 x 10 -10 M Learn more about KD
  • Storage bufferpH: 7.40
    Preservative: 0.01% Sodium azide
    Constituents: 50% Glycerol, 0.05% BSA
  • PurityTissue culture supernatant
  • ClonalityMonoclonal
  • Clone numberEPR6828(B)
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab125028 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 170 kDa (predicted molecular weight: 166 kDa).
ICC/IF Use a concentration of 1 µg/ml.
  • Application notesIs unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • FunctionMediates the endocytosis of glycoproteins by macrophages. Binds both sulfated and non-sulfated polysaccharide chains. Acts as phagocytic receptor for bacteria, fungi and other pathogens.
    • Sequence similaritiesContains 8 C-type lectin domains.
      Contains 1 fibronectin type-II domain.
      Contains 1 ricin B-type lectin domain.
    • Cellular localizationMembrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • bA541I19.1 antibody
      • C type lectin domain family 13 member D antibody
      • C-type lectin domain family 13 member D antibody
      • CD 206 antibody
      • CD206 antibody
      • CD206 antigen antibody
      • CLEC13D antibody
      • CLEC13DL antibody
      • Macrophage mannose receptor 1 antibody
      • Macrophage mannose receptor 1 like protein 1 antibody
      • Macrophage mannose receptor antibody
      • Mannose receptor C type 1 antibody
      • Mannose receptor C type 1 like 1 antibody
      • MMR antibody
      • MRC 1 antibody
      • MRC1 antibody
      • MRC1_HUMAN antibody
      • MRC1L1 antibody
      • OTTHUMP00000045206 antibody
      see all

    Anti-Mannose Receptor antibody [EPR6828(B)] images

    • All lanes : Anti-Mannose Receptor antibody [EPR6828(B)] (ab125028) at 1/1000 dilution

      Lane 1 : BxPC3 lysate
      Lane 2 : Caco2 lysate
      Lane 3 : HepG2 lysate
      Lane 4 : Human fetal liver lysate
      Lane 5 : 293T lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti rabbit at 1/2000 dilution

      Predicted band size : 166 kDa
    • Immunocytochemistry/Immunofluorescence analysis MOLT-4 (Human lymphoblastic leukemia) cells labeling Mannose Receptor with ab125028 at 1 μg/mL. Cells were fixed with 100% methanol. ab150081, an AlexaFluor®488 Goat anti-Rabbit at 1/1000 was used as the secondary antibody. Cells were counter-stained with ab195889 Anti-Alpha Tubulin antibody [DM1A] (1/250) - Microtubule Marker (Alexa Fluor® 594). Nuclei were counterstained with DAPI (blue).

      Confocal image showing membranous staining on MOLT-4 cell line.

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References for Anti-Mannose Receptor antibody [EPR6828(B)] (ab125028)

    This product has been referenced in:
    • Trial J  et al. Th1/M1 conversion to th2/m2 responses in models of inflammation lacking cell death stimulates maturation of monocyte precursors to fibroblasts. Front Immunol 4:287 (2013). ICC/IF ; Human . Read more (PubMed: 24065967) »

    See 1 Publication for this product

    Product Wall

    Application Western blot
    Sample Human Cell lysate - whole cell (Human Macrophages Type 2)
    Gel Running Conditions Reduced Denaturing (10)
    Loading amount 20 µg
    Specification Human Macrophages Type 2
    Blocking step Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 26°C
    Username

    Dr. FRANCESCO ELIA MARINO

    Verified customer

    Submitted Mar 21 2016

    Application Immunohistochemistry (Frozen sections)
    Sample Human Tissue sections (muscle)
    Specification muscle
    Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
    Fixative Formaldehyde
    Username

    Mr. Thomas Zwakhals

    Verified customer

    Submitted Mar 15 2016

    Application Immunohistochemistry (Frozen sections)
    Sample Rat Tissue sections (Brain)
    Permeabilization Yes - 0.1 M PBS with 3% Triton X
    Specification Brain
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
    Fixative Paraformaldehyde
    Username

    Dr. Ruma Raha-Chowdhury

    Verified customer

    Submitted Jun 25 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"