Overview

  • Product name
    Anti-Mannosidase II antibody
    See all Mannosidase II primary antibodies
  • Description
    Rabbit polyclonal to Mannosidase II
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 750 - 850 of Human Mannosidase II.

  • Positive control
    • This antibody gave a positive signal in the following tissue lysates: Human Skeletal Muscle; Human Liver; Human Placenta.

Properties

Applications

Our Abpromise guarantee covers the use of ab113448 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 131 kDa (predicted molecular weight: 131 kDa).

Target

  • Function
    Catalyzes the first committed step in the biosynthesis of complex N-glycans. It controls conversion of high mannose to complex N-glycans; the final hydrolytic step in the N-glycan maturation pathway.
  • Pathway
    Protein modification; protein glycosylation.
  • Sequence similarities
    Belongs to the glycosyl hydrolase 38 family.
  • Cellular localization
    Golgi apparatus membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 3-1 antibody
    • 6-alpha-mannosidase antibody
    • Alpha mannosidase 2 antibody
    • alpha Mannosidase II antibody
    • Alpha-mannosidase 2 antibody
    • AMAN II antibody
    • Golgi alpha mannosidase II antibody
    • Golgi alpha-mannosidase II antibody
    • Golgi integral membrane protein 7 antibody
    • GOLIM7 antibody
    • MA2A1_HUMAN antibody
    • MAN II antibody
    • Man2a1 antibody
    • MANA 2 antibody
    • MANA2 antibody
    • MANII antibody
    • Mann II antibody
    • Mannosidase alpha class 2A member 1 antibody
    • Mannosidase Two antibody
    • Mannosidase, alpha type II antibody
    • Mannosidase, alpha, II antibody
    • Mannosyl oligosaccharide 1 3 1 6 alpha mannosidase antibody
    • Mannosyl oligosaccharide 1,3 1,6 alpha mannosidase antibody
    • Mannosyl-oligosaccharide 1 antibody
    see all

Images

  • All lanes : Anti-Mannosidase II antibody (ab113448) at 1 µg/ml

    Lane 1 : Human skeletal muscle tissue lysate - total protein (ab29330)
    Lane 2 : Human liver tissue lysate - total protein (ab29889)
    Lane 3 : Human placenta tissue lysate - total protein (ab29745)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 131 kDa
    Observed band size : 131 kDa
    Additional bands at : 52 kDa,75 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 2 minutes

References

ab113448 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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