• Product nameAnti-Mannosidase II antibody
    See all Mannosidase II primary antibodies
  • Description
    Rabbit polyclonal to Mannosidase II
  • Tested applicationsSuitable for: ICCmore details
    Unsuitable for: IP or WB
  • Species reactivity
    Reacts with: Human, Monkey
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide to internal sequence (Human) conjugated to KLH.

  • Positive control
    • ICC: COS7 cells



Our Abpromise guarantee covers the use of ab12277 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC 1/200. Use with methanol fixed cells. 4% paraformaldehyde fixation reduces signal.
  • Application notesIs unsuitable for IP or WB.
  • Target

    • FunctionCatalyzes the first committed step in the biosynthesis of complex N-glycans. It controls conversion of high mannose to complex N-glycans; the final hydrolytic step in the N-glycan maturation pathway.
    • PathwayProtein modification; protein glycosylation.
    • Sequence similaritiesBelongs to the glycosyl hydrolase 38 family.
    • Cellular localizationGolgi apparatus membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • 3-1 antibody
      • 6-alpha-mannosidase antibody
      • Alpha mannosidase 2 antibody
      • alpha Mannosidase II antibody
      • Alpha-mannosidase 2 antibody
      • AMAN II antibody
      • Golgi alpha mannosidase II antibody
      • Golgi alpha-mannosidase II antibody
      • Golgi integral membrane protein 7 antibody
      • GOLIM7 antibody
      • MA2A1_HUMAN antibody
      • MAN II antibody
      • Man2a1 antibody
      • MANA 2 antibody
      • MANA2 antibody
      • MANII antibody
      • Mann II antibody
      • Mannosidase alpha class 2A member 1 antibody
      • Mannosidase Two antibody
      • Mannosidase, alpha type II antibody
      • Mannosidase, alpha, II antibody
      • Mannosyl oligosaccharide 1 3 1 6 alpha mannosidase antibody
      • Mannosyl oligosaccharide 1,3 1,6 alpha mannosidase antibody
      • Mannosyl-oligosaccharide 1 antibody
      see all

    Anti-Mannosidase II antibody images

    • COS7 cells were fixed with cold methanol for 10 minutes followed by incubation with ab12277 (1/200) in 5% normal goat serum. FITC-conjugated goat anti-rabbit was used to visualise bound rabbit antibodies. Nuclei were stained with DAPI.
    • ICC/IF image of ab12277 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab12277, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    References for Anti-Mannosidase II antibody (ab12277)

    This product has been referenced in:
    • Tian G  et al. An unconventional secretory pathway mediates the cilia targeting of peripherin/rds. J Neurosci 34:992-1006 (2014). Human . Read more (PubMed: 24431457) »
    • Ben Abdeljelil N  et al. The UL24 protein of herpes simplex virus 1 affects the sub-cellular distribution of viral glycoproteins involved in fusion. Virology 444:263-73 (2013). Read more (PubMed: 23876459) »

    See all 14 Publications for this product

    Product Wall

    Application Immunocytochemistry/ Immunofluorescence
    Sample Rat Cell (Rat hepatocytes)
    Permeabilization Yes - 0.2% Triton X-100
    Specification Rat hepatocytes
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
    Fixative Formaldehyde

    Dr. Armen Petrosyan

    Verified customer

    Submitted Aug 24 2015

    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HepG2 cells)
    Permeabilization Yes - 0.2% Triton X-100
    Specification HepG2 cells
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
    Fixative Formaldehyde

    Dr. Armen Petrosyan

    Verified customer

    Submitted Aug 24 2015

    Vielen Dank für Ihre Anfrage.
    Leider ist es nicht möglich die Konzentration von ab12277 und ab136497 zu bestimmen.
    Bei ab12277 und ab136497 handelt es sich um Vollserum.
    Die Konzentration von Antikörpern wird üblicher Weise über die Prot...

    Read More

    Thank you for your questions. I apologize for the delay in replying but I was unable to contact the laboratory that produces this antibody for information about FACS testing, or for more information about the immunogen.

    I expect that the ant...

    Read More