Overview

  • Product name
    Anti-Mannosidase II antibody
    See all Mannosidase II primary antibodies
  • Description
    Rabbit polyclonal to Mannosidase II
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IFmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    Synthetic peptide within Rat Mannosidase II aa 1120-1145 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.

  • Positive control
    • NRK cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab190774 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/500.

Cold methanol fixation is optimal as formaldehyde fixation leads to loss of signal.

  • Application notes
    Is unsuitable for WB.
  • Target

    • Function
      Catalyzes the first committed step in the biosynthesis of complex N-glycans. It controls conversion of high mannose to complex N-glycans; the final hydrolytic step in the N-glycan maturation pathway.
    • Pathway
      Protein modification; protein glycosylation.
    • Sequence similarities
      Belongs to the glycosyl hydrolase 38 family.
    • Cellular localization
      Golgi apparatus membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • 3-1 antibody
      • 6-alpha-mannosidase antibody
      • Alpha mannosidase 2 antibody
      • alpha Mannosidase II antibody
      • Alpha-mannosidase 2 antibody
      • AMAN II antibody
      • Golgi alpha mannosidase II antibody
      • Golgi alpha-mannosidase II antibody
      • Golgi integral membrane protein 7 antibody
      • GOLIM7 antibody
      • MA2A1_HUMAN antibody
      • MAN II antibody
      • Man2a1 antibody
      • MANA 2 antibody
      • MANA2 antibody
      • MANII antibody
      • Mann II antibody
      • Mannosidase alpha class 2A member 1 antibody
      • Mannosidase Two antibody
      • Mannosidase, alpha type II antibody
      • Mannosidase, alpha, II antibody
      • Mannosyl oligosaccharide 1 3 1 6 alpha mannosidase antibody
      • Mannosyl oligosaccharide 1,3 1,6 alpha mannosidase antibody
      • Mannosyl-oligosaccharide 1 antibody
      see all

    Images

    • Immunofluorescent analysis of methanol-fixed NRK cells labeling Mannosidase II with ab190774 at 1/100 dilution. FITC secondary antibody. Counter stained with DAPI (Blue).

    References

    ab190774 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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