The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.3 - 3 µg/ml. Detects a band of approximately 150 kDa (predicted molecular weight: 131 kDa).
Use at an assay dependent dilution.
FunctionCatalyzes the first committed step in the biosynthesis of complex N-glycans. It controls conversion of high mannose to complex N-glycans; the final hydrolytic step in the N-glycan maturation pathway.
PathwayProtein modification; protein glycosylation.
Sequence similaritiesBelongs to the glycosyl hydrolase 38 family.