Anti-Mannosidase II antibody (ab77353)

Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab77353 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3 - 3 µg/ml. Detects a band of approximately 150 kDa (predicted molecular weight: 131 kDa).
ELISA Use at an assay dependent dilution.

Target

  • FunctionCatalyzes the first committed step in the biosynthesis of complex N-glycans. It controls conversion of high mannose to complex N-glycans; the final hydrolytic step in the N-glycan maturation pathway.
  • PathwayProtein modification; protein glycosylation.
  • Sequence similaritiesBelongs to the glycosyl hydrolase 38 family.
  • Cellular localizationGolgi apparatus membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 3-1 antibody
    • 6-alpha-mannosidase antibody
    • Alpha mannosidase 2 antibody
    • alpha Mannosidase II antibody
    • Alpha-mannosidase 2 antibody
    • AMAN II antibody
    • Golgi alpha mannosidase II antibody
    • Golgi alpha-mannosidase II antibody
    • Golgi integral membrane protein 7 antibody
    • GOLIM7 antibody
    • MA2A1_HUMAN antibody
    • MAN II antibody
    • Man2a1 antibody
    • MANA 2 antibody
    • MANA2 antibody
    • MANII antibody
    • Mann II antibody
    • Mannosidase alpha class 2A member 1 antibody
    • Mannosidase Two antibody
    • Mannosidase, alpha type II antibody
    • Mannosidase, alpha, II antibody
    • Mannosyl oligosaccharide 1 3 1 6 alpha mannosidase antibody
    • Mannosyl oligosaccharide 1,3 1,6 alpha mannosidase antibody
    • Mannosyl-oligosaccharide 1 antibody
    see all

Anti-Mannosidase II antibody images

  • Anti-Mannosidase II antibody (ab77353) at 0.3 µg/ml + Human Placenta lysate (in RIPA buffer) at 35 µg

    Predicted band size : 131 kDa
    Observed band size : 150 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-Mannosidase II antibody (ab77353) at 3 µg/ml

    Lane 1 : MOCK-transfected HEK293 cell lysate
    Lane 2 : HEK293 cell lysate overexpressing Mannosidase II


    Predicted band size : 131 kDa

References for Anti-Mannosidase II antibody (ab77353)

ab77353 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (human dermal fibroblasts)
Loading amount 10 µg
Specification human dermal fibroblasts
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
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Submitted Aug 04 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"