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Anti-MAP 2a + 2b + 2c antibody [0.N.395] (ab33580)

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Overview

Product name

Anti-MAP 2a + 2b + 2c antibody [0.N.395]
See all MAP 2a + 2b + 2c products (3) ...

Description

Mouse monoclonal [0.N.395] to MAP 2a + 2b + 2c

Specificity

Ab33580 is highly specific to MAP 2a, b and c expressed in dendrites and cell bodies of neurons. It recognizes MAP2a and MAP2b (~280kD doublet) and MAP2c (70kD). It shows no crossreactivity with other MAPs, tau and tubulin.

Tested applications

IHC-P, IHC-FoFr, WB, ICC/IFmore details

Cross reactivity

Reacts with

Rat, Cow, Human, Xenopus laevis, Quail

Immunogen

Full length native protein: Bovine brain MAP2.

Epitope

Ab33580 reacts with an epitope located in in the first 151 amino acids of MAP2.

Positive control

IMR5 or T98G human glioblastoma cells or brain.

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 15mM Sodium Azide
Constituents: 0.2% BSA, 10mM PBS, pH 7.4

Concentration

Concentration information loading...

Purity

Protein G purified

Clonality

Monoclonal

Clone number

0.N.395

Isotype

IgG1

Research areas

Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microtubules >> MT Associated Proteins >> MAP
Neuroscience >> Cell Adhesion Proteins >> Cytoskeletal Proteins >> Microtubules

Applications

Show applications key

Our Abpromise guarantee covers the use of ab33580 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

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    IHC-P

     IHC-P: Use a concentrati...Read more →

    IHC-P: Use a concentration of 1 - 2 µg/ml.Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

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    IHC-FoFr

     IHC-FoFr: Use a concentr...Read more →

    IHC-FoFr: Use a concentration of 1 - 2 µg/ml.(Perform heat mediated antigen retrieval before commencing the IHC protocol.)

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    WB

     WB: Use a concentration ...Read more →

    WB: Use a concentration of 1 - 2 µg/ml.Predicted molecular weight: 199 kDa.

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    ICC/IF

     ICC/IF: Use at an assay ...Read more →

    ICC/IF: Use at an assay dependent dilution.

Target

Relevance

The expression of MAP2 is developmentally regulated and its multiple forms arise by alternative splicing of a single gene. MAP2 belongs to the microtubule associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The products of similar genes in rat and mouse are neuron specific cytoskeletal proteins that are enriched in dentrites, implicating a role in determining and stabilizing dentritic shape during neuron development. A number of alternatively spliced variants encoding distinct isforms have been described.

Cellular localization

Cytoplasm, cytoskeleton (Probable).

Alternative names

  • MAP 2 antibody
  • MAP2A antibody
  • MAP2B antibody
  • MAP2C antibody
  • MAP2R antibody
  • Microtubule associated protein 2 antibody
  • Mtap2 antibody
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Database links

References for Anti-MAP 2a + 2b + 2c antibody [0.N.395] (ab33580)

ab33580 has not yet been referenced specifically in any publications.

Publishing research using ab33580? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"