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Product Name
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MAP 2a + 2b + 2c antibody [0.N.395]
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See all MAP 2a + 2b + 2c antibodies (3)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [0.N.395] to MAP 2a + 2b + 2c
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Immunogen
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Full length native protein: Bovine brain MAP2.
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Reacts with
(species key)
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Hu, Rat, Cow, Ql, Xl
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Specificity
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Ab33580 is highly specific to MAP 2a, b and c expressed in dendrites and cell bodies of neurons. It recognizes MAP2a and MAP2b (~280kD doublet) and MAP2c (70kD). It shows no crossreactivity with other MAPs, tau and tubulin.
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Epitope
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Ab33580 reacts with an epitope located in in the first 151 amino acids of MAP2.
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Tested applications
(see key)
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ICC/IF, IHC-FoFr, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at an assay dependent dilution.
IHC-F: Use at a concentration of 1 - 2 µg/ml. Staining of formalin fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6 for 10-20 min., followed by cooling at room temperature for 20 min.
WB: Use at a concentration of 1 - 2 µg/ml. Predicted molecular weight: 199 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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IMR5 or T98G human glioblastoma cells or brain.
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Cellular localization
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Cytoplasm, cytoskeleton (Probable).
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Research areas
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Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microtubules >> MT Associated Proteins >> MAP
Neuroscience >> Cell Adhesion Proteins >> Cytoskeletal Proteins >> Microtubules
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Relevance
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The expression of MAP2 is developmentally regulated and its multiple forms arise by alternative splicing of a single gene. MAP2 belongs to the microtubule associated protein family. The proteins of this family are thought to be involved in microtubule assembly, which is an essential step in neurogenesis. The products of similar genes in rat and mouse are neuron specific cytoskeletal proteins that are enriched in dentrites, implicating a role in determining and stabilizing dentritic shape during neuron development. A number of alternatively spliced variants encoding distinct isforms have been described.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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0.N.395
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Isotype
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IgG1
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Purity
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Protein G purified
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Storage buffer
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Preservative: 15mM Sodium Azide
Constituents: 0.2% BSA, 10mM PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.200 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this MAP 2a + 2b + 2c antibody [0.N.395] is on this datasheet. But please do contact us if you would like any reassurance! |
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Search PubMed (MEDLINE) for references to MAP 2a + 2b + 2c
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