Overview

  • Product nameAnti-MAP kinase substrate 1 antibody [34E10]
  • Description
    Mouse monoclonal [34E10] to MAP kinase substrate 1
  • Specificityab27157 reacts specifically with MKS1 from plants as well as recombinant MKS1 expressed in E-Coli. Depending upon extract, other non-specific bands may occasionally be seen. The antibody recognizes MKS1 regardless of the phosphorylation status of MKS1.
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide - Selected peptide sequence from MKS1.

Properties

Applications

Our Abpromise guarantee covers the use of ab27157 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/50000.
WB 1/5000. Predicted molecular weight: 27 kDa.

Target

  • RelevanceMAP kinase substrate 1 (MKS1) is phosphorylated by MAP kinase 4 in Arabidopsis. MAP kinase 4 is involved in regulating plant defences against pathogens. It interacts with WRKY transcriptional regulators.
  • Cellular localizationNuclear
  • Alternative names
    • MKS 1 antibody
    • MKS1 antibody

Anti-MAP kinase substrate 1 antibody [34E10] images

  • All lanes : Anti-MAP kinase substrate 1 antibody [34E10] (ab27157) at 1/5000 dilution

    Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 2 : T24/83 (Human bladder carcinoma) Whole Cell Lysate
    Lane 3 : Saos 2 (Human epithelial-like osteosarcoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size : 27 kDa
    Observed band size : 24 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 140 kDa,42 kDa,48 kDa,70 kDa,75 kDa. We are unsure as to the identity of these extra bands.

References for Anti-MAP kinase substrate 1 antibody [34E10] (ab27157)

This product has been referenced in:

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