Overview

  • Product name
  • Description
    Rabbit polyclonal to MAP126
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human MAP126 aa 942-1193. The exact sequence is proprietary.
    Database link: Q96R06

  • Positive control
    • HeLa cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab184142 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Essential component of the mitotic spindle required for normal chromosome segregation and progression into anaphase. Required for chromosome alignment, normal timing of sister chromatid segregation, and maintenance of spindle pole architecture. The astrin (SPAG5)-kinastrin (SKAP) complex promotes stable microtubule-kinetochore attachments.
  • Tissue specificity
    Highly expressed in testis. Detected at low levels in placenta, liver, pancreas, thymus and colon.
  • Cellular localization
    Cytoplasm. Cytoplasm > cytoskeleton > spindle. Cytoplasm > cytoskeleton > spindle pole. Chromosome > centromere > kinetochore. In a punctate pattern in interphase cells. During mitosis, detected at spindle poles during prophase, throughout the spindle in metaphase and anaphase, and at midzone microtubules in anaphase and telophase. Detected on kinetochores of chromosomes that have congressed. The astrin (SPAG5)-kinastrin (SKAP) complex localizes to the microtubule plus ends.
  • Information by UniProt
  • Database links
  • Alternative names
    • Astrin antibody
    • Deepest antibody
    • hMAP 126 antibody
    • hMAP126 antibody
    • MAP 126 antibody
    • MAP126 antibody
    • Mitotic spindle associated protein antibody
    • Mitotic spindle associated protein p126 antibody
    • Mitotic spindle coiled coil related protein antibody
    • Mitotic spindle-associated protein p126 antibody
    • SPAG 5 antibody
    • SPAG5 antibody
    • SPAG5_HUMAN antibody
    • Sperm associated antigen 5 antibody
    • Sperm tail protein Spag 5 antibody
    • Sperm tail protein Spag5 antibody
    • Sperm-associated antigen 5 antibody
    see all

Images

  • Immunofluorescent analysis of HeLa cells (fixed in 100% methanol for 5 mins) labeling MAP126 with ab184142 at 1/500 dilution (green). Blue: Hoechst 33342 staining.

References

ab184142 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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