Overview

  • Product name
  • Description
    Rabbit polyclonal to MAP1A
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Cow, Dog, Pig, Gorilla
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Mouse MAP1A.

  • Positive control
    • This antibody gave a positive signal in Human Fetal Brain tissue lysate and HuES7 whole cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab101224 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 300 kDa (predicted molecular weight: 300 kDa).
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Function
    Structural protein involved in the filamentous cross-bridging between microtubules and other skeletal elements.
  • Tissue specificity
    Brain.
  • Sequence similarities
    Belongs to the MAP1 family.
  • Domain
    The basic region containing the repeats may be responsible for the binding of MAP1A to microtubules.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
    LC2 is generated from MAP1A by proteolytic processing.
  • Cellular localization
    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • MAP 1A antibody
    • MAP 1L antibody
    • MAP-1A antibody
    • MAP1 light chain LC2 antibody
    • Map1a antibody
    • MAP1A_HUMAN antibody
    • MAP1L antibody
    • Microtubule associated protein 1 like antibody
    • Microtubule associated protein 1A antibody
    • MTAP 1A antibody
    • MTAP1A antibody
    • Proliferation related protein p80 antibody
    • Proliferation-related protein p80 antibody
    see all

Images

  • All lanes : Anti-MAP1A antibody (ab101224) at 1 µg/ml

    Lane 1 : HUES7 (Human embryonic stem cell line) Whole Cell Lysate
    Lane 2 : Brain (Human) Tissue Lysate - fetal normal tissue (ab29467)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 300 kDa
    Observed band size : 300 kDa
    Additional bands at : 172 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 4 minutes
  • ab101224 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab101224 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

ab101224 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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