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Overview

  • Product name
    Anti-MAP1A antibody
    See all MAP1A primary antibodies
  • Description
    Rabbit polyclonal to MAP1A
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Cow, Dog, Pig, Gorilla
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Mouse MAP1A.

    Read Abcam's proprietary immunogen policy

  • Positive control
    • This antibody gave a positive signal in Human Fetal Brain tissue lysate and HuES7 whole cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab101224 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 300 kDa (predicted molecular weight: 300 kDa).
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Function
    Structural protein involved in the filamentous cross-bridging between microtubules and other skeletal elements.
  • Tissue specificity
    Brain.
  • Sequence similarities
    Belongs to the MAP1 family.
  • Domain
    The basic region containing the repeats may be responsible for the binding of MAP1A to microtubules.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
    LC2 is generated from MAP1A by proteolytic processing.
  • Cellular localization
    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • MAP 1A antibody
    • MAP 1L antibody
    • MAP-1A antibody
    • MAP1 light chain LC2 antibody
    • Map1a antibody
    • MAP1A_HUMAN antibody
    • MAP1L antibody
    • Microtubule associated protein 1 like antibody
    • Microtubule associated protein 1A antibody
    • MTAP 1A antibody
    • MTAP1A antibody
    • Proliferation related protein p80 antibody
    • Proliferation-related protein p80 antibody
    see all

Anti-MAP1A antibody images

  • Western blot - Anti-MAP1A antibody (ab101224)
    Western blot - Anti-MAP1A antibody (ab101224)
    All lanes : Anti-MAP1A antibody (ab101224) at 1 µg/ml

    Lane 1 : HUES7 (Human embryonic stem cell line) Whole Cell Lysate
    Lane 2 : Brain (Human) Tissue Lysate - fetal normal tissue (ab29467)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 300 kDa
    Observed band size : 300 kDa
    Additional bands at : 172 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 4 minutes
  • Immunocytochemistry/ Immunofluorescence - Anti-MAP1A antibody (ab101224)
    Immunocytochemistry/ Immunofluorescence - Anti-MAP1A antibody (ab101224)

    ab101224 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab101224 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-MAP1A antibody (ab101224)

ab101224 has not yet been referenced specifically in any publications.

Publishing research using ab101224? Please let us know so that we can cite the reference in this datasheet.

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