- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-MAP1A antibody
See all MAP1A primary antibodies -
DescriptionRabbit polyclonal to MAP1A
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Host speciesRabbit
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Tested applicationsSuitable for: WB, ICC/IFmore details
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Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Cow, Dog, Pig, Gorilla
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Immunogen
Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Mouse MAP1A.
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Positive control
- This antibody gave a positive signal in Human Fetal Brain tissue lysate and HuES7 whole cell lysate.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab101224 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 300 kDa (predicted molecular weight: 300 kDa). | |
| ICC/IF | Use a concentration of 5 µg/ml. |
Target
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FunctionStructural protein involved in the filamentous cross-bridging between microtubules and other skeletal elements.
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Tissue specificityBrain.
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Sequence similaritiesBelongs to the MAP1 family.
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DomainThe basic region containing the repeats may be responsible for the binding of MAP1A to microtubules.
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Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
LC2 is generated from MAP1A by proteolytic processing. -
Cellular localizationCytoplasm > cytoskeleton.
- Information by UniProt
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Database links
- Entrez Gene: 4130 Human
- Entrez Gene: 17754 Mouse
- Entrez Gene: 25152 Rat
- Omim: 600178 Human
- SwissProt: P78559 Human
- SwissProt: Q9QYR6 Mouse
- SwissProt: P34926 Rat
- Unigene: 194301 Human
see all -
Alternative names
- MAP 1A antibody
- MAP 1L antibody
- MAP-1A antibody
see all
Images
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Western blot - Anti-MAP1A antibody (ab101224)All lanes : Anti-MAP1A antibody (ab101224) at 1 µg/ml
Lane 1 : HUES7 (Human embryonic stem cell line) Whole Cell Lysate
Lane 2 : Brain (Human) Tissue Lysate - fetal normal tissue (ab29467)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 300 kDa
Observed band size: 300 kDa
Additional bands at: 172 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutes -
Immunocytochemistry/ Immunofluorescence - Anti-MAP1A antibody (ab101224)ab101224 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab101224 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References
ab101224 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"