Recombinant
RabMAb

Anti-MAP1A antibody [EPR18994] (ab184350)

Overview

  • Product name
    Anti-MAP1A antibody [EPR18994]
    See all MAP1A primary antibodies
  • Description
    Rabbit monoclonal [EPR18994] to MAP1A
  • Tested applications
    Suitable for: WB, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat
  • Immunogen

    Recombinant fragment within Mouse MAP1A aa 2550-2700. The exact sequence is proprietary.
    Database link: Q9QYR6

  • Positive control
    • WB: Mouse embryo, brain and spinal cord lysates; Rat brain lysate; Neuro-2a and C6 whole cell lysates. ICC/IF: Neuro-2a and C6 cells. Flow Cyt: Neuro-2a cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab184350 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 300, 26 kDa (predicted molecular weight: 305 kDa).
ICC/IF 1/1000.
Flow Cyt 1/150.

ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Structural protein involved in the filamentous cross-bridging between microtubules and other skeletal elements.
  • Tissue specificity
    Brain.
  • Sequence similarities
    Belongs to the MAP1 family.
  • Domain
    The basic region containing the repeats may be responsible for the binding of MAP1A to microtubules.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
    LC2 is generated from MAP1A by proteolytic processing.
  • Cellular localization
    Cytoplasm > cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • MAP 1A antibody
    • MAP 1L antibody
    • MAP-1A antibody
    • MAP1 light chain LC2 antibody
    • Map1a antibody
    • MAP1A_HUMAN antibody
    • MAP1L antibody
    • Microtubule associated protein 1 like antibody
    • Microtubule associated protein 1A antibody
    • MTAP 1A antibody
    • MTAP1A antibody
    • Proliferation related protein p80 antibody
    • Proliferation-related protein p80 antibody
    see all

Images

  • All lanes : Anti-MAP1A antibody [EPR18994] (ab184350) at 1/5000 dilution

    Lane 1 : Mouse embryo lysate
    Lane 2 : Mouse brain lysate
    Lane 3 : Rat brain lysate
    Lane 4 : Mouse spinal cord lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size : 305 kDa
    Observed band size : 26,300 kDa (why is the actual band size different from the predicted?)


    Exposure time : 15 seconds

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Anti-MAP1A antibody [EPR18994] (ab184350) at 1/1000 dilution + Neuro-2a (Mouse neuroblastoma cell line) whole cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Predicted band size : 305 kDa
    Observed band size : 26,300 kDa (why is the actual band size different from the predicted?)


    Exposure time : 30 seconds

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Anti-MAP1A antibody [EPR18994] (ab184350) at 1/2000 dilution + C6 (Rat glial tumor cell line) whole cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Predicted band size : 305 kDa
    Observed band size : 300 kDa (why is the actual band size different from the predicted?)


    Exposure time : 3 minutes

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Neuro-2a (Mouse neuroblastoma cell line) cells labeling MAP1A with ab184350 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on Neuro-2a cell line. The nuclear counter stain is DAPI (blue).

    Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) at 1/1000 dilution (red).

    The negative controls are as follows:
    -ve control 1: ab184350 at 1/1000 dilution followed by ab150120 at 1/1000 dilution.
    -ve control 2: ab7291  at 1/1000 dilution followed by ab150077  at 1/1000 dilution.

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized C6 (Rat glial tumor cell line) cells labeling MAP1A with ab184350 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on C6 cell line. The nuclear counter stain is DAPI (blue).

    Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed (ab150120) at 1/1000 dilution (red).

    The negative controls are as follows:
     -ve control 1: ab184350 at 1/1000 dilution followed by ab150120  at 1/1000 dilution.
     -ve control 2: ab7291 at 1/1000 dilution followed by ab150077  at 1/1000 dilution.

  • Flow cytometric analysis of 2% paraformaldehyde-fixed Neuro-2a (Mouse neuroblastoma cell line) cells labeling MAP1A with ab184350 at 1/150 dilution (red) compared with a Rabbit IgG,monoclonal  [EPR25A]- Isotype control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti Rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

References

ab184350 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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