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Product Name 

MAP1A antibody [HM-1]

 

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Product type 

Primary antibodies

Description 

Mouse monoclonal [HM-1] to MAP1A

Immunogen 

Rat brain MAPs preparation.

Reacts with 
(species key)

Ms, Rat

Specificity 

Ab11264 does not stain tubulin or other microtubule associated proteins.

Tested applications 
(see key)

IHC-Fr, WB


Abreviews 

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(see key)


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Application notes 
(see key)

Recommended dilutions
IHC-Fr: Use at an assay dependent dilution. Stains brain tissue showing selective labeling of neurons and stronger staining of axons and dendrites. Ab11264 stains both the cell bodies and dendritic trees of neurons throughout the brain. Purkinje cells are stained more strongly than granule cells.
WB: 1/500, this concentration was determined by indirect blotting using fresh total rat brain extract or enriched microtubule protein preparations. Predicted molecular weight: 350 kDa.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Whole brain homogenate.

Cellular localization 

Cytoplasmic

Research areas 

Neuroscience >> Neurology process >> Neurogenesis
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microtubules >> MT Associated Proteins >> MAP

Relevance 

MAP1A belongs to the microtubule-associated protein family which are involved in microtubule assembly. The precursor polypeptide product undergoes proteolytic processing to generate the final MAP1A heavy chain and LC2 light chain; these almost exclusively in the brain.

 

MAP1A antibody [HM-1] (ab11264)

Database links 

The links below go to external sites and will open in a new browser window

SwissProt

    

Q9QYR6    (Mouse)
P34926    (Rat)

Unigene

    

36501    (Mouse)
41412    (Rat)

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

HM-1

Isotype 

IgG1

Purity 

Ascites

Storage buffer 

Preservative: 15mM Sodium Azide
Constituents: Ascites

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

Batch dependent within range: 5.40 - 5.40 mg/ml

Concentration of lot no.     is  

Concentration not available for this lot.

Find concentration of your lot :

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Notes 

Storage in frost-free freezers is not recommended.
If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this MAP1A antibody [HM-1] is on this datasheet. But please do contact us if you would like any reassurance!


 

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