• Product nameAnti-MAP1B antibody [AA6]
    See all MAP1B primary antibodies
  • Description
    Mouse monoclonal [AA6] to MAP1B
  • SpecificityMonoclonal Anti-MAP1b does not react with tubulin of other Microtubule associated proteins.
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Chicken, Hamster, Cow, Cat, Human
  • Immunogen

    Rat brain microtubule associated proteins.

  • Positive control
    • Rat brain.
  • General notesStorage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.



Our Abpromise guarantee covers the use of ab11266 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notesICC/IF: Use at an assay dependent dilution in conjunction with either immunofluorescence or immunoperoxidase labelling methods.
    WB: 1/500. Predicted molecular weight: 270 kDa. May run higher due to many phosphorylation sites: 320kDa.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceMicrotubules are associated with a family of proteins called microtubule associated proteins (MAPs), which includes the protein t (tau) and a group of proteins referred to as MAP1, MAP2, MAP3, MAP4 and MAP5. MAP1B is the major microtubule associated protein in developing brain which changes its expression during development. In the newborn rat brain, it is a major component of microtubules but in the adult its level is ten fold lower. This change in the level of expression occurs simultaneously with neuronal maturation. MAP1B is the first MAP to appear in growing axons during development as it is present from the first emergence of the nascent axon from the cell body.
    • Cellular localizationCytoplasmic
    • Database links
    • Alternative names
      • FUTSCH antibody
      • LC1 antibody
      • MAP5 antibody
      • Microtubule associated protein 1B antibody
      • Mtap1b antibody
      • Mtap5 antibody
      see all

    Anti-MAP1B antibody [AA6] images

    • ab11266 (1/300) staining MAP1B (centre column) in Human cortical neurons by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% paraformaldehyde. Left column staining: tubulin, actin, synapsin, SAP102. Right column: overlay.

    References for Anti-MAP1B antibody [AA6] (ab11266)

    This product has been referenced in:
    • Eriksson M  et al. MAP1B binds to the NMDA receptor subunit NR3A and affects NR3A protein concentrations. Neurosci Lett 475:33-7 (2010). WB, ICC/IF ; Rat . Read more (PubMed: 20304030) »
    • Impens F  et al. Mechanistic insight into taxol-induced cell death. Oncogene 27:4580-91 (2008). WB ; Human . Read more (PubMed: 18408750) »

    See all 6 Publications for this product

    Product Wall

    Application Immunohistochemistry (PFA perfusion fixed frozen sections)
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 0.01M Citrate buffer pH6.0+Tween 0.05%
    Sample Mouse Tissue sections (brain)
    Specification brain
    Permeabilization Yes - 0.2%Triton X100
    Fixative Paraformaldehyde

    Abcam user community

    Verified customer

    Submitted Aug 08 2014

    Thank you for contacting us for technical support. I'm sorry to hear you are experiencing problems with your recent vial of ab11266. We have not received any other complaints about this very popular antibody. Could you please provide your order number ...

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    Thank you for your enquiry. I have received the following information from our source for this antibody, which I hope will be useful. I have added the publications listed below, along with the PubMed links, to the online datasheet for ab11266. "This...

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    Thank you for your enquiry. I have contacted our lab and I have discovered that given that this antibody was raised using rat brain microtubule associated proteins and unfortunately there has been no follow up to map the epitope. I also perform...

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    Thank you for your enquiry and your interest in our products. This antibody has been tested and characterized using newborn rat brain. Unfortunately, we have not particularly tested in Pericytes.