Overview

  • Product name
    Anti-MAP1B antibody [AA6]
    See all MAP1B primary antibodies
  • Description
    Mouse monoclonal [AA6] to MAP1B
  • Specificity
    Monoclonal Anti-MAP1b does not react with tubulin of other Microtubule associated proteins.
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Chicken, Hamster, Cow, Cat, Human
  • Immunogen

    Rat brain microtubule associated proteins.

  • Positive control
    • Rat brain.
  • General notes
    Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Properties

Applications

Our Abpromise guarantee covers the use of ab11266 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ICC/IF
  • Application notes
    ICC/IF: Use at an assay dependent dilution in conjunction with either immunofluorescence or immunoperoxidase labelling methods.
    WB: 1/500. Predicted molecular weight: 270 kDa. May run higher due to many phosphorylation sites: 320kDa.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Facilitates tyrosination of alpha-tubulin in neuronal microtubules (By similarity). Phosphorylated MAP1B may play a role in the cytoskeletal changes that accompany neurite extension. Possibly MAP1B binds to at least two tubulin subunits in the polymer, and this bridging of subunits might be involved in nucleating microtubule polymerization and in stabilizing microtubules. Acts as a positive cofactor in DAPK1-mediated autophagic vesicle formation and membrane blebbing.
    • Sequence similarities
      Belongs to the MAP1 family.
    • Domain
      Has a highly basic region with many copies of the sequence KKEE and KKEI/V, repeated but not at fixed intervals, which is responsible for the binding of MAP1B to microtubules.
    • Post-translational
      modifications
      LC1 is generated from MAP1B by proteolytic processing.
      S-nitrosylation at Cys-2464 enhances interaction with microtubules, and may act as an effector modification for neuronal nitric oxide synthase control of growth-cone size, growth-cone collapse and axon retraction.
    • Cellular localization
      Cytoplasm, cytoskeleton. Cytoplasm. Cell junction, synapse. Cell projection, dendritic spine. Colocalizes with DAPK1 in the microtubules and cortical actin fibers.
    • Information by UniProt
    • Database links
    • Alternative names
      • FUTSCH antibody
      • LC1 antibody
      • MAP-1B antibody
      • MAP1 light chain LC1 antibody
      • Map1b antibody
      • MAP1B_HUMAN antibody
      • MAP5 antibody
      • Microtubule associated protein 1B antibody
      • Mtap1b antibody
      • Mtap5 antibody
      see all

    Anti-MAP1B antibody [AA6] images

    • ab11266 (1/300) staining MAP1B (centre column) in Human cortical neurons by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% paraformaldehyde. Left column staining: tubulin, actin, synapsin, SAP102. Right column: overlay.

    References for Anti-MAP1B antibody [AA6] (ab11266)

    This product has been referenced in:
    • Eriksson M  et al. MAP1B binds to the NMDA receptor subunit NR3A and affects NR3A protein concentrations. Neurosci Lett 475:33-7 (2010). WB, ICC/IF ; Rat . Read more (PubMed: 20304030) »
    • Impens F  et al. Mechanistic insight into taxol-induced cell death. Oncogene 27:4580-91 (2008). WB ; Human . Read more (PubMed: 18408750) »

    See all 6 Publications for this product

    Product Wall

    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 0.01M Citrate buffer pH6.0+Tween 0.05%
    Sample
    Mouse Tissue sections (brain)
    Specification
    brain
    Permeabilization
    Yes - 0.2%Triton X100
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Aug 08 2014

    Thank you for contacting us for technical support. I'm sorry to hear you are experiencing problems with your recent vial of ab11266. We have not received any other complaints about this very popular antibody. Could you please provide your order number ...

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    Thank you for your enquiry. I have received the following information from our source for this antibody, which I hope will be useful. I have added the publications listed below, along with the PubMed links, to the online datasheet for ab11266. "This...

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    Thank you for your enquiry. I have contacted our lab and I have discovered that given that this antibody was raised using rat brain microtubule associated proteins and unfortunately there has been no follow up to map the epitope. I also perform...

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    Thank you for your enquiry and your interest in our products. This antibody has been tested and characterized using newborn rat brain. Unfortunately, we have not particularly tested in Pericytes.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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