Overview

  • Product name
  • Description
    Rabbit polyclonal to MAP1B
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 1824-1878 of Human MAP1B (UniProt: P46821).

  • Positive control
    • U-87 MG, P4.5 Mouse brain lysate, NT2D1, IMR32 and Neuro-2a whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab154333 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 271 kDa.

Target

  • Function
    Facilitates tyrosination of alpha-tubulin in neuronal microtubules (By similarity). Phosphorylated MAP1B may play a role in the cytoskeletal changes that accompany neurite extension. Possibly MAP1B binds to at least two tubulin subunits in the polymer, and this bridging of subunits might be involved in nucleating microtubule polymerization and in stabilizing microtubules. Acts as a positive cofactor in DAPK1-mediated autophagic vesicle formation and membrane blebbing.
  • Sequence similarities
    Belongs to the MAP1 family.
  • Domain
    Has a highly basic region with many copies of the sequence KKEE and KKEI/V, repeated but not at fixed intervals, which is responsible for the binding of MAP1B to microtubules.
  • Post-translational
    modifications
    LC1 is generated from MAP1B by proteolytic processing.
    S-nitrosylation at Cys-2464 enhances interaction with microtubules, and may act as an effector modification for neuronal nitric oxide synthase control of growth-cone size, growth-cone collapse and axon retraction.
  • Cellular localization
    Cytoplasm, cytoskeleton. Cytoplasm. Cell junction, synapse. Cell projection, dendritic spine. Colocalizes with DAPK1 in the microtubules and cortical actin fibers.
  • Information by UniProt
  • Database links
  • Alternative names
    • FUTSCH antibody
    • LC1 antibody
    • MAP-1B antibody
    • MAP1 light chain LC1 antibody
    • Map1b antibody
    • MAP1B_HUMAN antibody
    • MAP5 antibody
    • Microtubule associated protein 1B antibody
    • Mtap1b antibody
    • Mtap5 antibody
    see all

Images

  • Anti-MAP1B antibody (ab154333) at 1/1000 dilution + U-87 MG whole cell lysate at 30 µg

    Predicted band size: 271 kDa



    5% SDS PAGE
  • Anti-MAP1B antibody (ab154333) at 1/1000 dilution + P4.5 Mouse brain lysate at 15 µg

    Predicted band size: 271 kDa



    5% SDS PAGE

References

ab154333 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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