Anti-MAP1LC3A antibody - N-terminal (ab173700)

Overview

  • Product name
    Anti-MAP1LC3A antibody - N-terminal
    See all MAP1LC3A primary antibodies
  • Description
    Rabbit polyclonal to MAP1LC3A - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human MAP1LC3A aa 1-30 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q9H492

  • Positive control
    • Rat brain lysate (membrane fraction and soluble fraction); Mouse brain tissue lysate; U251 cells; Wild-type cerebellar cells; Human brain tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab173700 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500. Predicted molecular weight: 14 kDa.
ICC/IF 1/10 - 1/50.

Target

  • Function
    Probably involved in formation of autophagosomal vacuoles (autophagosomes).
  • Tissue specificity
    Most abundant in heart, brain, liver, skeletal muscle and testis but absent in thymus and peripheral blood leukocytes.
  • Sequence similarities
    Belongs to the MAP1 LC3 family.
  • Post-translational
    modifications
    The precursor molecule is cleaved by APG4B/ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II.
  • Cellular localization
    Cytoplasm > cytoskeleton. Endomembrane system. Cytoplasmic vesicle > autophagosome membrane. LC3-II binds to the autophagic membranes.
  • Information by UniProt
  • Database links
  • Alternative names
    • ATG8E antibody
    • Autophagy-related protein LC3 A antibody
    • Autophagy-related ubiquitin-like modifier LC3 A antibody
    • LC3 antibody
    • LC3A antibody
    • MAP1 light chain 3 like protein 1 antibody
    • MAP1 light chain 3-like protein 1 antibody
    • MAP1A/1B light chain 3 A antibody
    • MAP1A/MAP1B LC3 A antibody
    • MAP1A/MAP1B light chain 3 A antibody
    • MAP1ALC3 antibody
    • MAP1BLC3 antibody
    • Map1lc3a antibody
    • Microtubule associated proteins 1A/1B light chain 3 antibody
    • Microtubule-associated protein 1 light chain 3 alpha antibody
    • Microtubule-associated proteins 1A and 1B, light chain 3 antibody
    • Microtubule-associated proteins 1A/1B light chain 3A antibody
    • MLP3A_HUMAN antibody
    see all

Images

  • All lanes : Anti-MAP1LC3A antibody - N-terminal (ab173700) at 1/100 dilution

    Lane 1 : Rat brain lysate, membrane fraction
    Lane 2 : Rat brain lysate, soluble fraction

    Lysates/proteins at 12.5 µg per lane.

    Predicted band size: 14 kDa



    lipidated MAP1LC3A (arrow, II) and non-lipidated MAP1LC3A (arrow, I)

  • All lanes : Anti-MAP1LC3A antibody - N-terminal (ab173700) at 1/100 dilution

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Mouse brain tissue lysate with blocking peptide

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 14 kDa

  • Immunofluorescent analysis of U251 cells labeling MAP1LC3A with ab173700. U251 cells were treated with Chloroquine (50 μM,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with ab173700 (1/200, 2 h at room temperature). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1/1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min).

  • Immunofluorescent analysis of Wild-type and mutant cerebellar cells labeling MAP1LC3A with ab173700 at 1/10 dilution (Red). Co-immunostaining of CLN3-encoded protein, battenin with a monoclonal anti-battenin antibody (Green). DAPI staining (Blue).

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human brain tissue labeling MAP1LC3A with ab173700 at 1/50 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.

References

ab173700 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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