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Product Name
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MAP2 antibody [AA6]
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See all MAP2 antibodies (14)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [AA6] to MAP2
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Immunogen
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Rat brain taxol stabilized microtubules.
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Reacts with
(species key)
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Mamm
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions WB: 1/1000. Predicted molecular weight: 280 kDa (MAP2 is made up of two ~280kDa apparent molecular weight bands referred to as MAP2a and MAP2b. A third lower molecular weight form, usually called MAP2c, corresponds to a pair of protein bands running at ~70kDa on SDS-PAGE gels).
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Cytoplasmic. Cytoskeleton.
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Research areas
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Stem Cells >> Neural Stem Cells >> Neuron Restricted Lineage Neuroscience >> Cell Type Marker >> Neuron marker >> Dendrite marker Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microtubules >> MT Associated Proteins >> MAP Neuroscience >> Cell Type Marker >> Neuron marker >> Soma marker
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Relevance
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MAP2 is the major microtubule associated protein of brain tissue. There are three forms of MAP2; two are similarily sized with apparent molecular weights of 280 kDa (MAP2a and MAP2b) and the third with a lower molecular weight of 70 kDa (MAP2c). In the newborn rat brain, MAP2b and MAP2c are present, while MAP2a is absent. Between postnatal days 10 and 20, MAP2a appears. At the same time, the level of MAP2c drops by 10-fold. This change happens during the period when dendrite growth is completed and when neurons have reached their mature morphology. MAP2 is degraded by a Cathepsin D-like protease in the brain of aged rats. There is some indication that MAP2 is expressed at higher levels in some types of neurons than in other types. MAP2 is known to promote microtubule assembly and to form side-arms on microtubules. It also interacts with neurofilaments, actin, and other elements of the cytoskeleton.
This antibody will stain neuronal cell bodies and dendritic processes, and will not stain glial cells or axons. It is the best marker to distinguish dendrites from axons in the brain.
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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AA6
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Isotype
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IgG2a
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Purity
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Protein A purified
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Storage buffer
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Preservative: 0.03% Thimerosal (merthiolate) Constituents: PBS Material safety datasheet (MSDS) for this product: Thimerosal MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this MAP2 antibody [AA6] is on this datasheet. But please do contact us if you would like any reassurance! |
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Search PubMed (MEDLINE) for references to MAP2
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