Overview

  • Product name
  • Description
    Rabbit polyclonal to MAP2
  • Tested applications
    Suitable for: IHC-P, IHC-Fr, IHC-FoFr, IHC (PFA fixed), ICC, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Goat, Cat, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Rat MAP2.

    (Peptide available as ab32453.)

  • Positive control
    • Brain (Mouse) Whole Cell Lysate - normal tissue, 0 days old, Brain (Rat) Whole Cell Lysate - normal tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab32454 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr 1/200 - 1/400.
IHC-FoFr Use at an assay dependent concentration. PubMed: 19540881
IHC (PFA fixed) Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 250 kDa.Can be blocked with Rat MAP2 peptide (ab32453).

Target

  • Function
    The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.
  • Sequence similarities
    Contains 3 Tau/MAP repeats.
  • Post-translational
    modifications
    Phosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules, and their disassembly (By similarity). Isoform 2 is probably phosphorylated by PKA at Ser-323, Ser-354 and Ser-386 and by FYN at Tyr-67.
  • Cellular localization
    Cytoplasm, cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp686I2148 antibody
    • MAP 2 antibody
    • MAP dendrite specific antibody
    • MAP-2 antibody
    • MAP2 antibody
    • MAP2A antibody
    • MAP2B antibody
    • MAP2C antibody
    • Microtubule associated protein 2 antibody
    • Microtubule-associated protein 2 antibody
    • MTAP2_HUMAN antibody
    see all

Images

  • ab32454 staining MAP2 in mouse brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/3000 in blocking buffer) for 2 hours at 21°C in TBS/BSA/azide. An undiluted Biotin-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

  • IHC-P image of ab32454 stained lizard spinal cord. Tissue was fixed in formaldehyde with heat mediated antigen retrieval using citric acid. Tissue was blocked for 10 minutes in 1% B.S.A, and incubated with the antibody (ab32454, 1/250) for 2 hours at 21°C. The secondary antibody used was a goat-anti rabbit IgG conjugated to bitoin (1/250).

    See Abreview

  • ICC/IF image of ab32454 stained rat PC12 cells. The cells were PFA fixed (10 min), permabilised in PBS-T (20 min) and incubated with the antibody (ab32454, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

  • ab32454 staining MAP2 in rat cryopreserved embryonic cortical neurons cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde with picric acid. Samples were incubated with primary antibody (1/2000 in 10mM PBS + 0.3% Triton-X) for 12 hours at 4°C. An Alexa Fluor® 488-conjugated donkey anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.

    See Abreview

  • IHC image of MAP2 staining in human cerebral cortex FFPE section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab32454, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • All lanes : Anti-MAP2 antibody (ab32454) at 1 µg/ml

    Lane 1 : Mouse brain tissue lysate - total protein (0 days) (ab7188)
    Lane 2 : Brain (Rat) Tissue Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 70, 199, 280 kDa
    Observed band size : 260,280 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 110 kDa,199 kDa,65 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes

References

This product has been referenced in:
  • Poulsen ET  et al. An Aberrant Phosphorylation of Amyloid Precursor Protein Tyrosine Regulates Its Trafficking and the Binding to the Clathrin Endocytic Complex in Neural Stem Cells of Alzheimer's Disease Patients. Front Mol Neurosci 10:59 (2017). ICC/IF . Read more (PubMed: 28360834) »
  • Wenger A  et al. Stem cell cultures derived from pediatric brain tumors accurately model the originating tumors. Oncotarget 8:18626-18639 (2017). ICC/IF ; Human . Read more (PubMed: 28148893) »

See all 45 Publications for this product

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Dog Cell (Induced neurons from bone marrow derived mesenchym)
Permeabilization
Yes - Triton-X100
Specification
Induced neurons from bone marrow derived mesenchym
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 26°C
Fixative
Paraformaldehyde
Username

清隆 新井

Verified customer

Submitted Sep 19 2016

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Spinal cord)
Antigen retrieval step
None
Permeabilization
Yes - Triton-X100
Specification
Spinal cord
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 26°C
Fixative
Paraformaldehyde
Username

清隆 新井

Verified customer

Submitted Sep 14 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (PC12)
Permeabilization
Yes - Triton-X100
Specification
PC12
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 26°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Sep 14 2016

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (induced neuron derived from pluripotent cells)
Permeabilization
Yes - 0.25% Triton X-100 in PBS
Specification
induced neuron derived from pluripotent cells
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Dec 16 2015

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (neuron)
Permeabilization
Yes - 0.2%Triton
Specification
neuron
Blocking step
Serum as blocking agent for 10 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Oct 12 2015

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Brain cell culture)
Specification
Brain cell culture
Blocking step
BSA as blocking agent for 13 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Oct 12 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Horse Tissue sections (Cerebrum)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate Buffer
Permeabilization
No
Specification
Cerebrum
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 24°C
Fixative
Formaldehyde
Username

清隆 新井

Verified customer

Submitted Sep 30 2015

Application
Flow Cytometry
Sample
Human Cell (Differentiated hNSCs)
Permeabilization
Yes - 0.25% Triton X-100 in DPBS
Gating Strategy
Undifferentiated Stem Cells (white)
Specification
Differentiated hNSCs
Preparation
Cell harvesting/tissue preparation method: Accutase
Sample buffer: PBS
Fixation
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Sep 09 2015

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Brain)
Permeabilization
Yes - Triton x-100, 0.01%
Specification
Brain
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 1%
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Jun 18 2015

Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Permeabilization
Yes - Triton x-100, 0.01%
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Mar 24 2015

1-10 of 34 Abreviews or Q&A

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