FunctionThe exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.
Sequence similaritiesContains 3 Tau/MAP repeats.
Post-translational modificationsPhosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules, and their disassembly (By similarity). Isoform 2 is probably phosphorylated by PKA at Ser-323, Ser-354 and Ser-386 and by FYN at Tyr-67.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody (ab32454)This image is courtesy of an Abreview by Carl Hobbs.
ab32454 staining MAP2 in mouse brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/3000 in blocking buffer) for 2 hours at 21°C in TBS/BSA/azide. An undiluted Biotin-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAP2 antibody (ab32454)Image courtesy of Carl Hobbs, Kings College London, U.K
IHC-P image of ab32454 stained lizard spinal cord. Tissue was fixed in formaldehyde with heat mediated antigen retrieval using citric acid. Tissue was blocked for 10 minutes in 1% B.S.A, and incubated with the antibody (ab32454, 1/250) for 2 hours at 21°C. The secondary antibody used was a goat-anti rabbit IgG conjugated to bitoin (1/250).
ICC/IF image of ab32454 stained rat PC12 cells. The cells were PFA fixed (10 min), permabilised in PBS-T (20 min) and incubated with the antibody (ab32454, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
Immunocytochemistry/ Immunofluorescence - Anti-MAP2 antibody (ab32454)This image is courtesy of an Abreview submitted by Babben Tinner
ab32454 staining MAP2 in rat cryopreserved embryonic cortical neurons cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde with picric acid. Samples were incubated with primary antibody (1/2000 in 10mM PBS + 0.3% Triton-X) for 12 hours at 4°C. An Alexa Fluor® 488-conjugated donkey anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.
IHC image of MAP2 staining in human cerebral cortex FFPE section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab32454, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blot - MAP2 antibody - Neuronal Marker (ab32454)
All lanes : Anti-MAP2 antibody (ab32454) at 1 µg/ml
Lane 1 : Mouse brain tissue lysate - total protein (0 days) (ab7188) Lane 2 : Brain (Rat) Tissue Lysate
Lysates/proteins at 20 µg per lane.
Secondary Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution