Overview

  • Product nameAnti-MAP2 antibody
    See all MAP2 primary antibodies
  • Description
    Rabbit polyclonal to MAP2
  • Tested applicationsSuitable for: IHC-P, IHC-Fr, IHC-FoFr, IHC (PFA fixed), ICC, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Goat, Cat, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Rat MAP2.

    (Peptide available as ab32453.)

  • Positive control
    • Brain (Mouse) Whole Cell Lysate - normal tissue, 0 days old, Brain (Rat) Whole Cell Lysate - normal tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab32454 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr 1/200 - 1/400.
IHC-FoFr Use at an assay dependent concentration. PubMed: 19540881
IHC (PFA fixed) Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 250 kDa.Can be blocked with Rat MAP2 peptide (ab32453).

Target

Anti-MAP2 antibody images

  • ab32454 staining MAP2 in mouse brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/3000 in blocking buffer) for 2 hours at 21°C in TBS/BSA/azide. An undiluted Biotin-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

  • IHC-P image of ab32454 stained lizard spinal cord. Tissue was fixed in formaldehyde with heat mediated antigen retrieval using citric acid. Tissue was blocked for 10 minutes in 1% B.S.A, and incubated with the antibody (ab32454, 1/250) for 2 hours at 21°C. The secondary antibody used was a goat-anti rabbit IgG conjugated to bitoin (1/250).

    See Abreview

  • ICC/IF image of ab32454 stained rat PC12 cells. The cells were PFA fixed (10 min), permabilised in PBS-T (20 min) and incubated with the antibody (ab32454, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

  • ab32454 staining MAP2 in rat cryopreserved embryonic cortical neurons cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde with picric acid. Samples were incubated with primary antibody (1/2000 in 10mM PBS + 0.3% Triton-X) for 12 hours at 4°C. An Alexa Fluor® 488-conjugated donkey anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.

    See Abreview

  • IHC image of MAP2 staining in human cerebral cortex FFPE section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab32454, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • All lanes : Anti-MAP2 antibody (ab32454) at 1 µg/ml

    Lane 1 : Mouse brain tissue lysate - total protein (0 days) (ab7188)
    Lane 2 : Brain (Rat) Tissue Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 70, 199, 280 kDa
    Observed band size : 260,280 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 110 kDa,199 kDa,65 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes

References for Anti-MAP2 antibody (ab32454)

This product has been referenced in:
  • Shigyo M  et al. Extracellular vimentin interacts with insulin-like growth factor 1 receptor to promote axonal growth. Sci Rep 5:12055 (2015). IF ; Rat . Read more (PubMed: 26170015) »
  • Kirby ED  et al. Adult hippocampal neural stem and progenitor cells regulate the neurogenic niche by secreting VEGF. Proc Natl Acad Sci U S A 112:4128-33 (2015). Read more (PubMed: 25775598) »

See all 22 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Dog Cell (Induced neurons from bone marrow derived mesenchym)
Permeabilization Yes - Triton-X100
Specification Induced neurons from bone marrow derived mesenchym
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 26°C
Fixative Paraformaldehyde
Username

清隆 新井

Verified customer

Submitted Sep 19 2016

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Rat Tissue sections (Spinal cord)
Antigen retrieval step None
Permeabilization Yes - Triton-X100
Specification Spinal cord
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 26°C
Fixative Paraformaldehyde
Username

清隆 新井

Verified customer

Submitted Sep 14 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (PC12)
Permeabilization Yes - Triton-X100
Specification PC12
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 26°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Sep 14 2016

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (induced neuron derived from pluripotent cells)
Permeabilization Yes - 0.25% Triton X-100 in PBS
Specification induced neuron derived from pluripotent cells
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative Paraformaldehyde
Username

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Verified customer

Submitted Dec 16 2015

Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (neuron)
Permeabilization Yes - 0.2%Triton
Specification neuron
Blocking step Serum as blocking agent for 10 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative Formaldehyde
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Submitted Oct 12 2015

Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Brain cell culture)
Specification Brain cell culture
Blocking step BSA as blocking agent for 13 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative Paraformaldehyde
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Submitted Oct 12 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Horse Tissue sections (Cerebrum)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate Buffer
Permeabilization No
Specification Cerebrum
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 24°C
Fixative Formaldehyde
Username

清隆 新井

Verified customer

Submitted Sep 30 2015

Application Flow Cytometry
Sample Human Cell (Differentiated hNSCs)
Permeabilization Yes - 0.25% Triton X-100 in DPBS
Gating Strategy Undifferentiated Stem Cells (white)
Specification Differentiated hNSCs
Preparation Cell harvesting/tissue preparation method: Accutase
Sample buffer: PBS
Fixation Paraformaldehyde
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Submitted Sep 09 2015

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (Brain)
Permeabilization Yes - Triton x-100, 0.01%
Specification Brain
Blocking step (agent) for 1 hour(s) and 0 minute(s) · Concentration: 1%
Fixative Paraformaldehyde
Username

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Submitted Jun 18 2015

Application Immunohistochemistry (Frozen sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Sample Mouse Tissue sections (Brain)
Specification Brain
Permeabilization Yes - Triton x-100, 0.01%
Fixative Paraformaldehyde
Username

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Verified customer

Submitted Mar 24 2015

1-10 of 34 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"