Overview

  • Product nameAnti-MAP2 antibody
    See all MAP2 primary antibodies
  • Description
    Chicken polyclonal to MAP2
  • Tested applicationsSuitable for: ELISA, IHC-Fr, IHC-FoFr, IHC-P, WB, ICC/IF, IHC (PFA fixed)more details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human, Cynomolgus Monkey, Marmoset (common), Aplysia
  • Immunogen

    Full length native protein (purified) corresponding to Cow MAP2.

  • Positive control
    • ICC: cultured rat cortical neurons

Properties

Applications

Our Abpromise guarantee covers the use of ab5392 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/5000.
IHC-Fr Use at an assay dependent dilution.
IHC-FoFr 1/500. PubMed: 18684835
IHC-P Use at an assay dependent concentration.
WB 1/100000. This 280kDa band corresponds to the high molecular weight MAP2a and MAP2b isoforms. Also detects 2 bands at approximately 70 kDa, corresponding to MAPc, since the 70kDa bands are transcripts from the same gene and correspond to the C-terminus of the 280kDa bands.
ICC/IF 1/10000.
IHC (PFA fixed) Use at an assay dependent concentration.

Target

Anti-MAP2 antibody images

  • ab5392 at a dilution of 1/10000, staining MAP 2 (green) in tissue cultured rat cortical neurons by immunofluorescence. Nuclei are stained blue with Dapi.
  • Rat cortical neurons and glia in mixed tissue culture stained with ab5392 (Chicken antibody to MAP2) (green)(1:30 000), a mouse monoclonal antibody to GFAP (red) and nuclei of all cells stained with Hoechst dye (blue).
  • ab5392 staining MAP2 in rat spinal cord tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with paraformaldehyde and permeablized with 50% Ethanol for 30 minutes. The sample was incubated with primary antibody (1/250 in PBS + 0.3% Triton X-100) at 4°C for 48 hours. An Alexa Fluor® 488-conjugated goat anti-chicken IgY polyclonal (1/1000) was used as the secondary antibody.

    See Abreview

  • ab5392 at 1/5000 staining PFA fixed free floating sections of mouse brain.  An AlexaFluor® 633 conjugated goat anti-chicken antibody was used as the secondary.

    See Abreview

  • ab5392 staining MAP2 in mouse neurons by immunocytochemistry/ immunofluorescence. Cells were PFA fixed and permeabilized in 0.4% Triton X-100 prior to blocking in 5% serum for 1 hour at 25°C. The primary antibody was diluted 1/10000 and incubated with the sample for 20 hours at 21°C. Alexa fluor® 488 goat polyclonal to chicken IgY, diluted 1/400, was used as the secondary antibody.

    See Abreview

  • ab5392 staining MAP2 in murine brain tissue sections by Immunohistochemistry (PFA fixed).
    Boiling in citrate-buffer was used as antigen retrieval method. ab5392 used at a 1/2000 dilution for 12 hours. The secondary used was an Alexa-Fluor 555 conjugated goat anti-chicken polyclonal used at a 1/400 dilution.

    See Abreview

  • ab5392 detecting MAP2 recombinant protein by direct ELISA. Mouse recombinat protien was coated on to microplate in carbonate coating buffer pH 9.6 for 1 hour at 37°C, Plate were blocked with 3% BSA for 1 hour at 37°C and incubated with the primary antibody (1/5000 in PBS + 1% Tween-20) for 1 hour at 37°C. An alkaline phosphatase Goat anti-chicken IgG polyclonal (1/100000) was used as the secondary antibody.

    See Abreview

  • Anti-MAP2 antibody (ab5392) at 1/10000 dilution + Mouse brain lysate - post nuclear supernatant at 10 µg

    Secondary
    HRP-conjugated rabbit anti-chicken IgY at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Observed band size : 70,280 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

    This image is courtesy of an anonymous Abreview

    See Abreview

References for Anti-MAP2 antibody (ab5392)

This product has been referenced in:
  • Vessoni AT  et al. Cockayne syndrome-derived neurons display reduced synapse density and altered neural network synchrony. Hum Mol Genet 25:1271-80 (2016). Read more (PubMed: 26755826) »
  • Kawanai T  et al. Prenatal Exposure to Histone Deacetylase Inhibitors Affects Gene Expression of Autism-Related Molecules and Delays Neuronal Maturation. Neurochem Res 41:2574-2584 (2016). Read more (PubMed: 27300699) »

See all 79 Publications for this product

Product Wall

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Antigen retrieval step None
Sample Rat Tissue sections (Spinal cord)
Specification Spinal cord
Permeabilization Yes - 50% Ethanol 30 min
Fixative Paraformaldehyde
Username

Mr. Tom Owen

Verified customer

Submitted Apr 08 2015

Application Immunocytochemistry/ Immunofluorescence
Sample Horse Cell (Olfactory stem cells)
Permeabilization Yes - 0,1% Triton X100
Specification Olfactory stem cells
Blocking step BSA 3% + Goat serum 5% as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Nov 18 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Dog Cell (Olfactory stem cells)
Permeabilization Yes - 0,1% Triton X100
Specification Olfactory stem cells
Blocking step BSA 3% + Goat serum 5% as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative Paraformaldehyde
Username

Dr. Antoine Veron

Verified customer

Submitted Nov 18 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (Olfactory stem cells GFP)
Permeabilization Yes - 0,1% Triton X100
Specification Olfactory stem cells GFP
Blocking step BSA 3% + Goat serum 5% as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Nov 18 2016

Application Immunocytochemistry
Sample Mouse Cultured Cells (NSCs (Neural Stem cells))
Permeabilization Yes - tritonx100
Specification NSCs (Neural Stem cells)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Fixative Paraformaldehyde
Username

Ms. kyungjoo seong

Verified customer

Submitted Nov 02 2016

Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (Neuron)
Permeabilization Yes - TX100 0.05%
Specification Neuron
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Jun 20 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Ferret Tissue sections (P0-ferret-parietal cortex)
Permeabilization Yes - 0.5% Triton in 1x PBS for 1 hour5% Triton in 1x PBS for 1 hour
Specification P0-ferret-parietal cortex
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative Paraformaldehyde
Username

Francis Djankpa

Verified customer

Submitted Apr 29 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Ferret Tissue sections (parietal cortex in Brain coronal slices)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 1x Citrate Buffer
Permeabilization Yes - 0.5% Triton in 1x PBS for 1 hour
Specification parietal cortex in Brain coronal slices
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 23°C
Fixative Paraformaldehyde
Username

Francis Djankpa

Verified customer

Submitted Mar 03 2016

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rabbit Tissue sections (Ear base)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer pH 6
Permeabilization No
Specification Ear base
Blocking step Protein Block serum free from Dako as blocking agent as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 37°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

Submitted Dec 09 2015

Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Whole Brain Organotypic Slice Cultures)
Permeabilization Yes - 0.3% Triton x-100 in PBS
Specification Whole Brain Organotypic Slice Cultures
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Nov 11 2015

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