Anti-MAP2 antibody (ab75713)

Overview

  • Product name
  • Description
    Chicken polyclonal to MAP2
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Two synthetic peptidescorresponding to different regions of the MAP2 gene product, but shared between the human (NP_002365, NCBI) and mouse (P20357, NCBI) sequences conjugated to KLH.

  • Positive control
    • Cultured neurons from an e13.5 mouse brain.
  • General notes


    Two different affinity purified anti peptide antibodies were combined to make this product. The concentrations of both of these antibodies was 100 µg/ml, making the total antibody concentration 200 µg/ml.

Properties

Applications

Our Abpromise guarantee covers the use of ab75713 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/5000. Predicted molecular weight: 200 kDa.
IHC-P 1/1000 - 1/2000.
ICC/IF 1/1000 - 1/2000.

Target

  • Function
    The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.
  • Sequence similarities
    Contains 3 Tau/MAP repeats.
  • Post-translational
    modifications
    Phosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules, and their disassembly (By similarity). Isoform 2 is probably phosphorylated by PKA at Ser-323, Ser-354 and Ser-386 and by FYN at Tyr-67.
  • Cellular localization
    Cytoplasm, cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp686I2148 antibody
    • MAP 2 antibody
    • MAP dendrite specific antibody
    • MAP-2 antibody
    • MAP2 antibody
    • MAP2A antibody
    • MAP2B antibody
    • MAP2C antibody
    • Microtubule associated protein 2 antibody
    • Microtubule-associated protein 2 antibody
    • MTAP2_HUMAN antibody
    see all

Anti-MAP2 antibody images

  • Immunocytochemistry/Immunofluorescence analysis of cultured neonatal mouse cortex cells labelling MAP2 (green) with ab75713. Cells were fixed with 4% paraformaldehyde. Red - rabbit anti-GFAP.

  • Immunocytochemistry/Immunofluorescence analysis of cultured neonatal mouse cortex cells labelling MAP2 (green) with ab75713. Cells were fixed with 4% paraformaldehyde. Red - rabbit anti-GFAP, Blue - DAPI nuclear stain.

References for Anti-MAP2 antibody (ab75713)

ab75713 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us and thank you for submitting an abreview. The abreview program is only for testing primary antibodies in untested species/assays. Unfortunately it does not include secondary antibodies. However, it looks as the seconday anti...

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Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Fixative
Acetone
Permeabilization
Yes - 0.3% Triton X-100/PBS
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Dr. Julie Nigro

Verified customer

Submitted Dec 28 2011

Thank you for submitting an Abreview of ab56164. As you may have noticed, your review has now been published on our website. We appreciate the time and effort that you have put into this and would like to express our gratitude. If there is anything tha...

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