Overview

  • Product nameAnti-MAP2 antibody
    See all MAP2 primary antibodies
  • Description
    Chicken polyclonal to MAP2
  • Tested applicationsSuitable for: WB, ICC/IF, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human
  • Immunogen

    Recombinant full length protein corresponding to Cow MAP2. Recombinant bovine MAP2 protein expressed in and purified from E. Coli

  • Positive control
    • Rat cortex lysate; mixed neuron/glial cultures.

Properties

Applications

Our Abpromise guarantee covers the use of ab92434 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/20000. Predicted molecular weight: 280 kDa.
ICC/IF 1/2500.
ICC Use at an assay dependent concentration.

Target

Anti-MAP2 antibody images

  • ab92434 staining MAP2 in mouse brain neurons by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 5% serum for 1 hour at 20°C. Samples were incubated with primary antibody (1/2500 in PBS) for 24 hours at 4°C. ab150169, a goat anti-chicken Alexa Fluor® 488 (1/500) was used as the secondary antibody.

    See Abreview

  • ab92434 at 1/2500 dilution staining MAP2 in mixed neuron/glial cultures. by Immunofluorescence. The perikarya and dendrites of neurons are strongly and specifically stained with the MAP2 antibody (red). Cell nuclei are visualized with DAPI DNA stain.
  • Anti-MAP2 antibody (ab92434) at 1/20000 dilution + Rat cerebral cortex lysate

    Predicted band size : 280 kDa

References for Anti-MAP2 antibody (ab92434)

This product has been referenced in:
  • Derlig K  et al. Simiate is an Actin binding protein involved in filopodia dynamics and arborization of neurons. Front Cell Neurosci 8:99 (2014). Human . Read more (PubMed: 24782708) »
  • Soetedjo L  et al. Targeting of vasoactive intestinal peptide receptor 2, VPAC2, a secretin family G-protein coupled receptor, to primary cilia. Biol Open 2:686-94 (2013). ICC/IF . Read more (PubMed: 23862016) »

See all 4 Publications for this product

Product Wall

Application Immunocytochemistry
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Sample Mouse Cell (Brain, neurons)
Specification Brain, neurons
Permeabilization No
Fixative Paraformaldehyde
Username

Ms. Kirsty Ireland

Verified customer

Submitted Mar 19 2014

Thank you for contacting us.

For this chicken polyclonal MAP2 antibody the concentration is roughly between 15 and 30µg per 100µl, however, we have not determined the absolute concentration. This is because some antibodies can have high conc...

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Vielen Dank für Ihre Antwort und für diese weiteren Informationen. Es tut mir wirklich leid zu hören, dass die MAP2- und Cy5-Antikörper in der Immunfluoreszenz mit PFA-Fixierung nicht funktionieren. Beide Antikörper haben unsere strikten Qualitätsk...

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Vielen Dank für Ihren Anruf. Es tut mir leid zu hören, dass Sie Probleme mit unserem MAP2-Antikörper ab92434 haben. Wie besprochen habe ich unseren Fragebogen als Word-Dokument an diese E-Mail angehängt. Durch das Ausfüllen des Fragebogens erhalten ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"