Overview

  • Product name
  • Description
    Chicken polyclonal to MAP2
  • Tested applications
    Suitable for: WB, ICC/IF, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human
  • Immunogen

    Recombinant full length protein corresponding to Cow MAP2. Recombinant bovine MAP2 protein expressed in and purified from E. Coli

  • Positive control
    • Rat cortex lysate; mixed neuron/glial cultures.

Properties

Applications

Our Abpromise guarantee covers the use of ab92434 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/20000. Predicted molecular weight: 280 kDa.
ICC/IF 1/2500.
ICC Use at an assay dependent concentration.

Target

  • Function
    The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.
  • Sequence similarities
    Contains 3 Tau/MAP repeats.
  • Post-translational
    modifications
    Phosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules, and their disassembly (By similarity). Isoform 2 is probably phosphorylated by PKA at Ser-323, Ser-354 and Ser-386 and by FYN at Tyr-67.
  • Cellular localization
    Cytoplasm, cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp686I2148 antibody
    • MAP 2 antibody
    • MAP dendrite specific antibody
    • MAP-2 antibody
    • MAP2 antibody
    • MAP2A antibody
    • MAP2B antibody
    • MAP2C antibody
    • Microtubule associated protein 2 antibody
    • Microtubule-associated protein 2 antibody
    • MTAP2_HUMAN antibody
    see all

Images

  • ab92434 staining MAP2 in mouse brain neurons by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 5% serum for 1 hour at 20°C. Samples were incubated with primary antibody (1/2500 in PBS) for 24 hours at 4°C. ab150169, a goat anti-chicken Alexa Fluor® 488 (1/500) was used as the secondary antibody.

    See Abreview

  • Anti-MAP2 antibody (ab92434) at 1/20000 dilution + Rat cerebral cortex lysate

    Predicted band size : 280 kDa
  • ab92434 at 1/2500 dilution staining MAP2 in mixed neuron/glial cultures. by Immunofluorescence. The perikarya and dendrites of neurons are strongly and specifically stained with the MAP2 antibody (red). Cell nuclei are visualized with DAPI DNA stain.

References

This product has been referenced in:
  • Sellgren CM  et al. Patient-specific models of microglia-mediated engulfment of synapses and neural progenitors. Mol Psychiatry 22:170-177 (2017). ICC/IF ; Human . Read more (PubMed: 27956744) »
  • Westbroek W  et al. A new glucocerebrosidase-deficient neuronal cell model provides a tool to probe pathophysiology and therapeutics for Gaucher disease. Dis Model Mech 9:769-78 (2016). ICC . Read more (PubMed: 27482815) »

See all 7 Publications for this product

Customer reviews and Q&As

Application
Immunocytochemistry
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Sample
Mouse Cell (Brain, neurons)
Specification
Brain, neurons
Permeabilization
No
Fixative
Paraformaldehyde
Username

Ms. Kirsty Ireland

Verified customer

Submitted Mar 19 2014

Thank you for contacting us.

For this chicken polyclonal MAP2 antibody the concentration is roughly between 15 and 30µg per 100µl, however, we have not determined the absolute concentration. This is because some antibodies can have high conc...

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Vielen Dank für Ihre Antwort und für diese weiteren Informationen. Es tut mir wirklich leid zu hören, dass die MAP2- und Cy5-Antikörper in der Immunfluoreszenz mit PFA-Fixierung nicht funktionieren. Beide Antikörper haben unsere strikten Qualitätsk...

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Vielen Dank für Ihren Anruf. Es tut mir leid zu hören, dass Sie Probleme mit unserem MAP2-Antikörper ab92434 haben. Wie besprochen habe ich unseren Fragebogen als Word-Dokument an diese E-Mail angehängt. Durch das Ausfüllen des Fragebogens erhalten ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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