Overview

  • Product name
    Anti-MAP2 antibody [HM-2]
    See all MAP2 primary antibodies
  • Description
    Mouse monoclonal [HM-2] to MAP2
  • Specificity
    Monoclonal Anti-MAP2 does not react with tubulin of other microtubule associated proteins. This antibody recognizes all forms of MAP2 (namely MAP2a, b and c) in human, rat, mouse, bovine, chicken, and quail.
  • Tested applications
    Suitable for: ICC, IHC-Fr, ICC/IF, IHC-FrFl, WB, IHC-P, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Chicken, Cow, Human, Quail
  • Immunogen

    Other Immunogen Type corresponding to MAP2. HM-2 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with rat brain microtubule associated proteins (MAPs).

  • Positive control
    • Rat brain extract.
  • General notes
    Storage in frost-free freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Properties

Applications

Our Abpromise guarantee covers the use of ab11267 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ICC/IF 1/500. Fix cells in 4% paraformaldehyde/PBS for 45 min; then permeabilise cells with 0.2% Triton X-100 in PBS for 5 min (see Farah et al) OR fix in 4% paraformaldehyde (containing 0.2% picric acid in 0.1 M phosphate buffer, pH 6.9) for 15 min at room temperature (see O' Hare et al) .
IHC-FrFl Use at an assay dependent concentration. PubMed: 24223856
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 280 kDa. (see Farah et al).
IHC-P Use at an assay dependent concentration.
IHC-FoFr 1/500. PubMed: 20424326

Target

  • Function
    The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.
  • Sequence similarities
    Contains 3 Tau/MAP repeats.
  • Post-translational
    modifications
    Phosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules, and their disassembly (By similarity). Isoform 2 is probably phosphorylated by PKA at Ser-323, Ser-354 and Ser-386 and by FYN at Tyr-67.
  • Cellular localization
    Cytoplasm, cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp686I2148 antibody
    • MAP 2 antibody
    • MAP dendrite specific antibody
    • MAP-2 antibody
    • MAP2 antibody
    • MAP2A antibody
    • MAP2B antibody
    • MAP2C antibody
    • Microtubule associated protein 2 antibody
    • Microtubule-associated protein 2 antibody
    • MTAP2_HUMAN antibody
    see all

Anti-MAP2 antibody [HM-2] images

  • ab11267 staining MAP2 in human hippocampal progenitor cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.3% Triton X-100 and blocked with 5% serum for 1 hour at 18°C. Samples were incubated with the undiluted primary antibody for 12 hours at 4°C. An undiluted Alexa Fluor® 555-conjugated donkey anti-mouse IgG polyclonal was used as the secondary antibody.

    See Abreview

  • Immunocytochemical analysis of B35 cells labelling MAP2 with ab11267 at a concentration of 2 μg/mL. The secondary was developed using Goat anti-mouse IgG. Cells were counterstained with DAPI to stain nuclei.

  • ab11267 staining rat brain tissue sections by IHC-Fr.  Sections were PFA fixed and permeabilized in TritonX100 prior to blocking with 3% BSA for 1 hour.  The rpimary antibody was diluted 1/500 and incubated with the sample for 12 hours at 4°C.  An Alexa Fluor® conjugated goat anti-mouse was used as the secondary antibody. 

    The image shows a cross section through the rat hipocampal CA1 area at magnification 200x . The anti-MAP2 staining is clearly visible in dendrites of pyramidal cells.

    See Abreview

References for Anti-MAP2 antibody [HM-2] (ab11267)

This product has been referenced in:
  • Loss O & Stephenson FA Developmental changes in trak-mediated mitochondrial transport in neurons. Mol Cell Neurosci 80:134-147 (2017). Rat . Read more (PubMed: 28300646) »
  • Kobayashi S  et al. Local Somatodendritic Translation and Hyperphosphorylation of Tau Protein Triggered by AMPA and NMDA Receptor Stimulation. EBioMedicine 20:120-126 (2017). Read more (PubMed: 28566250) »

See all 52 Publications for this product

Product Wall

Application
Immunocytochemistry
Sample
Rat Cell (Cortical neurons from E17 embryos)
Permeabilization
Yes - 0.2% Triton X 5 mins
Specification
Cortical neurons from E17 embryos
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Jun 12 2015

Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse Neuroblastoma (Neuro2A))
Gel Running Conditions
Reduced Denaturing (8)
Loading amount
400000 cells
Treatment
10% FBS and fibroblast growth factor
Specification
Mouse Neuroblastoma (Neuro2A)
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 28°C
Username

Abcam user community

Verified customer

Submitted Apr 27 2015

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C
Sample
Human Cell (hippocampal progenitor cells)
Specification
hippocampal progenitor cells
Permeabilization
Yes - 0.3% Triton X-100
Fixative
Paraformaldehyde
Username

Dr. Aleksandra Maruszak

Verified customer

Submitted Mar 31 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Mouse Cultured Cells (Neural stem cells and progenitors)
Specification
Neural stem cells and progenitors
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.3% Triton-X-100 for 30 min
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Nov 02 2012

I am sorry that these antibodies did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact our accounting department so that we ...

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Thanks for your reply. I am very sorry these anti-GFAP and anti-MAP2 antibodies continue to give you trouble. I'd be happy to send you any two replacement antibodies from our catalog. Or if you prefer I can set up a credit or refund instead. Please let...

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We spoke just a short while ago regarding these issues you were having with ab90601 and ab11267. I have processed the replacement orders as we discussed and sent you an update via email just a few minutes ago. You should receive these 2 replacement ant...

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I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab90601 and one vial of ab11267. To check the status of the order ple...

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Thank you for contacting us. I am sorry that ab11267 did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement. To check the status of the order please contact our...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry free floating
Sample
Mouse Tissue sections (Adult C57BL/6 mouse coronal brain sections)
Specification
Adult C57BL/6 mouse coronal brain sections
Username

Mr. Xiao-Dong Wang

Verified customer

Submitted Aug 03 2010

1-10 of 13 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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