Recombinant
RabMAb

Anti-MAP2 (phospho S136) antibody [EPR2361] (ab96378)

Overview

  • Product name
    Anti-MAP2 (phospho S136) antibody [EPR2361]
    See all MAP2 primary antibodies
  • Description
    Rabbit monoclonal [EPR2361] to MAP2 (phospho S136)
  • Tested applications
    Suitable for: WB, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human MAP2 (phospho S136).

  • Positive control
    • WB: Human fetal brain and mouse brain tissue lysates IHC-P: Human brain tissue
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab96378 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 200 kDa.
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function
      The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.
    • Sequence similarities
      Contains 3 Tau/MAP repeats.
    • Post-translational
      modifications
      Phosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules, and their disassembly (By similarity). Isoform 2 is probably phosphorylated by PKA at Ser-323, Ser-354 and Ser-386 and by FYN at Tyr-67.
    • Cellular localization
      Cytoplasm, cytoskeleton.
    • Information by UniProt
    • Database links
    • Alternative names
      • DKFZp686I2148 antibody
      • MAP 2 antibody
      • MAP dendrite specific antibody
      • MAP-2 antibody
      • MAP2 antibody
      • MAP2A antibody
      • MAP2B antibody
      • MAP2C antibody
      • Microtubule associated protein 2 antibody
      • Microtubule-associated protein 2 antibody
      • MTAP2_HUMAN antibody
      see all

    Images

    • All lanes : Anti-MAP2 (phospho S136) antibody [EPR2361] (ab96378) at 1/1000 dilution

      Lane 1 : Untreated fetal brain lysates
      Lane 2 : Fetal brain lysates treated with Alkaline Phosphatase
      Lane 3 : Mouse brain lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP/AP polymerized antibody

      Predicted band size : 200 kDa
    • ab96378, at a 1/100 dilution, staining MAP2 in paraffin embedded Human brain tissue by Immunohistochemical analysis.

    References

    This product has been referenced in:
    • Ni N  et al. Effects of let-7b and TLX on the proliferation and differentiation of retinal progenitor cells in vitro. Sci Rep 4:6671 (2014). ICC ; Mouse . Read more (PubMed: 25327364) »

    See 1 Publication for this product

    Customer reviews and Q&As

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rat Cell (Cryopreserved embryonic cortical neurons (QBMcells)
    Specification
    Cryopreserved embryonic cortical neurons (QBMcells
    Permeabilization
    No
    Fixative
    paraformaldehyde with picric acid
    Username

    Ms. Babben Tinner

    Verified customer

    Submitted Feb 19 2015

    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Sample
    Zebrafish Tissue sections (Retina, Inner plexiform layer)
    Specification
    Retina, Inner plexiform layer
    Permeabilization
    Yes - triton X
    Fixative
    Paraformaldehyde
    Username

    Dr. Ryan Macdonald

    Verified customer

    Submitted Nov 17 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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