The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-Fr: 1/500. WB: Use at an assay dependent dilution. Detects a band of approximately 300 kDa. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
MAP2 is the major microtubule associated protein of brain tissue. There are three forms of MAP2; two are similarily sized with apparent molecular weights of 280 kDa (MAP2a and MAP2b) and the third with a lower molecular weight of 70 kDa (MAP2c). The expression of MAP2 is developmentally regulated and its multiple forms arise by alternative splicing of a single gene.
References for Anti-MAP2a + MAP2b antibody [AP20] (FITC) (ab24279)
This product has been referenced in:
Schober et al. Microfluidics and biosensors as tools for NanoBioSystems research with applications in the "Life Science" Materials Science and Engineering: B169:174-181 (2009).
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Guzman R et al. Long-term monitoring of transplanted human neural stem cells in developmental and pathological contexts with MRI. Proc Natl Acad Sci U S A104:10211-6 (2007).
Read more (PubMed: 17553967) »