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Full length native protein (purified) Microtubule protein enriched for kinesin
Our Abpromise guarantee covers the use of ab36447 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
|ICC||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 2 - 5 µg/ml. Predicted molecular weight: 200 kDa.|
|IHC-Fr||Use at an assay dependent concentration.|
|IHC-P||Use a concentration of 10 µg/ml.|
|ICC/IF||Use at an assay dependent concentration.|
ab36447 staining MAP2a + MAP2b in Rat cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with Formaldehyde + Methanol, permeabilized and blocked with 10% serum for 60 minutes at 21°C. Samples were incubated with primary antibody (1/400 in PBS) for 16 hours at 4°C. An Alexa Fluor®488-conjugated Goat anti-mouse polyclonal (1/800) was used as the secondary antibody.
ab36447 staining MAP2a + MAP2b in Mouse brain tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3.6% Vector MOM kit mouse Ig blocking reagent for 1 hour at 20°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/200) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated Donkey anti-mouse polyclonal (1/1000) was used as the secondary antibody.
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