Anti-MAP2a + MAP2b antibody [MT-07] (ab36447)

Overview

  • Product nameAnti-MAP2a + MAP2b antibody [MT-07]
    See all MAP2a + MAP2b primary antibodies
  • Description
    Mouse monoclonal [MT-07] to MAP2a + MAP2b
  • Specificityab36447 recognizes Microtubule associated protein 2ab (MAP2ab).
  • Tested applicationsSuitable for: ELISA, IP, ICC, WB, IHC-Fr, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Pig
  • Immunogen

    Full length native protein (purified) Microtubule protein enriched for kinesin

  • Positive control
    • Porcine brain, Mouse brain, human neuroblastoma.

Properties

Applications

Our Abpromise guarantee covers the use of ab36447 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
ICC Use a concentration of 5 µg/ml.
WB Use a concentration of 2 - 5 µg/ml. Predicted molecular weight: 200 kDa.
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

Target

  • RelevanceMAP2 is the major microtubule associated protein of brain tissue. There are three forms of MAP2; two are similarily sized with apparent molecular weights of 280 kDa (MAP2a and MAP2b) and the third with a lower molecular weight of 70 kDa (MAP2c). The expression of MAP2 is developmentally regulated and its multiple forms arise by alternative splicing of a single gene.
  • Cellular localizationCytoplasm, cytoskeleton
  • Database links
  • Alternative names
    • MAP2 antibody
    • MAP2A antibody
    • MAP2B antibody
    • Microtubule associated protein 2 antibody

Anti-MAP2a + MAP2b antibody [MT-07] images

  • ab36447 staining MAP2a + MAP2b in Rat cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with Formaldehyde + Methanol, permeabilized and blocked with 10% serum for 60 minutes at 21°C. Samples were incubated with primary antibody (1/400 in PBS) for 16 hours at 4°C. An Alexa Fluor®488-conjugated Goat anti-mouse polyclonal (1/800) was used as the secondary antibody.

    See Abreview

References for Anti-MAP2a + MAP2b antibody [MT-07] (ab36447)

ab36447 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (Primary hippocampal neurons)
Specification Primary hippocampal neurons
Fixative Formaldehyde+Methanol
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Ms. Jen-Ling Wang

Verified customer

Submitted Apr 05 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"