Overview

  • Product name
    Anti-MAP2a + MAP2b antibody [MT-07]
    See all MAP2a + MAP2b primary antibodies
  • Description
    Mouse monoclonal [MT-07] to MAP2a + MAP2b
  • Specificity
    ab36447 recognizes Microtubule associated protein 2ab (MAP2ab).
  • Tested applications
    Suitable for: ELISA, IP, ICC, WB, IHC-Fr, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Pig
  • Immunogen

    Full length native protein (purified) Microtubule protein enriched for kinesin

  • Positive control
    • Porcine brain, Mouse brain, human neuroblastoma.

Properties

Applications

Our Abpromise guarantee covers the use of ab36447 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
ICC Use a concentration of 5 µg/ml.
WB Use a concentration of 2 - 5 µg/ml. Predicted molecular weight: 200 kDa.
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

Target

  • Relevance
    MAP2 is the major microtubule associated protein of brain tissue. There are three forms of MAP2; two are similarily sized with apparent molecular weights of 280 kDa (MAP2a and MAP2b) and the third with a lower molecular weight of 70 kDa (MAP2c). The expression of MAP2 is developmentally regulated and its multiple forms arise by alternative splicing of a single gene.
  • Cellular localization
    Cytoplasm, cytoskeleton
  • Database links
  • Alternative names
    • MAP2 antibody
    • MAP2A antibody
    • MAP2B antibody
    • Microtubule associated protein 2 antibody

Images

  • ab36447 staining MAP2a + MAP2b in Rat cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with Formaldehyde + Methanol, permeabilized and blocked with 10% serum for 60 minutes at 21°C. Samples were incubated with primary antibody (1/400 in PBS) for 16 hours at 4°C. An Alexa Fluor®488-conjugated Goat anti-mouse polyclonal (1/800) was used as the secondary antibody.

    See Abreview

References

This product has been referenced in:
  • Oikari LE  et al. Cell surface heparan sulfate proteoglycans as novel markers of human neural stem cell fate determination. Stem Cell Res 16:92-104 (2016). ICC/IF ; Human . Read more (PubMed: 26722758) »
  • Oikari LE  et al. Data defining markers of human neural stem cell lineage potential. Data Brief 7:206-15 (2016). ICC/IF ; Human . Read more (PubMed: 26958640) »

See all 2 Publications for this product

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Mouse brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer
Permeabilization
No
Specification
Mouse brain
Blocking step
Vector MOM kit mouse Ig blocking reagent as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3.6% · Temperature: 20°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Feb 22 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Mouse neurons, in vitro differentiated)
Permeabilization
Yes - 0.2% TX-100/PBS
Specification
Mouse neurons, in vitro differentiated
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Feb 14 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Primary hippocampal neurons)
Specification
Primary hippocampal neurons
Fixative
Formaldehyde+Methanol
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Ms. Jen-Ling Wang

Verified customer

Submitted Apr 05 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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