Overview

  • Product nameAnti-MAP2K1IP1 antibody
    See all MAP2K1IP1 primary antibodies
  • Description
    Mouse monoclonal to MAP2K1IP1
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment: MADDLKRFLY KKLPSVEGLH AIVVSDRDGV PVIKVANDNA PEHALRPGFL STFALATDQG SKLGLSKNKS IICYYNTYQV VQFNRLP, corresponding to amino acids 1-87 of human MAP2K1IP1 with a 26 kDa tag

  • Positive control
    • Immunogen; MAP2K1IP1 transfected 293T cell line

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • IsotypeIgG1
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab76984 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 14 kDa.
ELISA Use at an assay dependent dilution. Detection limit for recombinant tagged MAP2K1IP1 is approximately 10 ng/ml if used as a capture antibody.

Target

  • FunctionAs part of the Ragulator complex it is involved in amino acid sensing and activation of mTORC1, a signaling complex promoting cell growth in response to growth factors, energy levels, and amino acids. Activated by amino acids through a mechanism involving the lysosomal V-ATPase, the Ragulator functions as a guanine nucleotide exchange factor activating the small GTPases Rag. Activated Ragulator and Rag GTPases function as a scaffold recruiting mTORC1 to lysosomes where it is in turn activated. Adapter protein that enhances the efficiency of the MAP kinase cascade facilitating the activation of MAPK2.
  • Sequence similaritiesBelongs to the LAMTOR3 family.
  • Cellular localizationLate endosome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • LAMTOR3 antibody
    • Late endosomal/lysosomal adaptor and MAPK and MTOR activator 3 antibody
    • LTOR3_HUMAN antibody
    • MAP2K1IP1 antibody
    • MEK binding partner 1 antibody
    • MEK partner 1 antibody
    • MEK-binding partner 1 antibody
    • Mitogen activated protein kinase kinase 1 interacting protein 1 antibody
    • Mitogen-activated protein kinase kinase 1-interacting protein 1 antibody
    • Mitogen-activated protein kinase scaffold protein 1 antibody
    • Mp1 antibody
    • Ragulator complex protein LAMTOR3 antibody
    see all

Anti-MAP2K1IP1 antibody images

  • Anti-MAP2K1IP1 antibody (ab76984) at 5 µg/ml + immunogen at 0.2 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 14 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-MAP2K1IP1 antibody (ab76984) at 5 µg/ml

    Lane 1 : MAP2K1IP1 transfected 293T cell line
    Lane 2 : non transfected 293T cell line

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 14 kDa
    Observed band size : 10 kDa (why is the actual band size different from the predicted?)

References for Anti-MAP2K1IP1 antibody (ab76984)

ab76984 has not yet been referenced specifically in any publications.

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