Anti-MAP2K1IP1 antibody (ab96653)

Overview

  • Product nameAnti-MAP2K1IP1 antibody
    See all MAP2K1IP1 primary antibodies
  • Description
    Rabbit polyclonal to MAP2K1IP1
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Zebrafish
  • Immunogen

    Recombinant fragment, corresponding to amino acids 1-124 of Human MAP2K1IP1

  • Positive control
    • Paraffin-embedded SW480 xenograft. 293T whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab96653 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 14 kDa.
IHC-P 1/100 - 1/500.

Target

  • FunctionAs part of the Ragulator complex it is involved in amino acid sensing and activation of mTORC1, a signaling complex promoting cell growth in response to growth factors, energy levels, and amino acids. Activated by amino acids through a mechanism involving the lysosomal V-ATPase, the Ragulator functions as a guanine nucleotide exchange factor activating the small GTPases Rag. Activated Ragulator and Rag GTPases function as a scaffold recruiting mTORC1 to lysosomes where it is in turn activated. Adapter protein that enhances the efficiency of the MAP kinase cascade facilitating the activation of MAPK2.
  • Sequence similaritiesBelongs to the LAMTOR3 family.
  • Cellular localizationLate endosome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • LAMTOR3 antibody
    • Late endosomal/lysosomal adaptor and MAPK and MTOR activator 3 antibody
    • LTOR3_HUMAN antibody
    • MAP2K1IP1 antibody
    • MEK binding partner 1 antibody
    • MEK partner 1 antibody
    • MEK-binding partner 1 antibody
    • Mitogen activated protein kinase kinase 1 interacting protein 1 antibody
    • Mitogen-activated protein kinase kinase 1-interacting protein 1 antibody
    • Mitogen-activated protein kinase scaffold protein 1 antibody
    • Mp1 antibody
    • Ragulator complex protein LAMTOR3 antibody
    see all

Anti-MAP2K1IP1 antibody images

  • Anti-MAP2K1IP1 antibody (ab96653) at 1/1000 dilution + 293T whole cell lysate at 30 µg

    Predicted band size : 14 kDa
  • ab96653, at 1/500, staining paraffin-embedded SW480 xenograft by Immunohistochemistry.

References for Anti-MAP2K1IP1 antibody (ab96653)

ab96653 has not yet been referenced specifically in any publications.

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (bladder cancer cells)
Loading amount 20 µg
Specification bladder cancer cells
Gel Running Conditions Non-reduced Denaturing (15)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
Username

Dr. santosh chauhan

Verified customer

Submitted Aug 23 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"