The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/10000 - 1/50000. Detects a band of approximately 14 kDa.
Use at an assay dependent concentration.
1/50 - 1/100.
Application notesIs unsuitable for Flow Cyt.
FunctionAs part of the Ragulator complex it is involved in amino acid sensing and activation of mTORC1, a signaling complex promoting cell growth in response to growth factors, energy levels, and amino acids. Activated by amino acids through a mechanism involving the lysosomal V-ATPase, the Ragulator functions as a guanine nucleotide exchange factor activating the small GTPases Rag. Activated Ragulator and Rag GTPases function as a scaffold recruiting mTORC1 to lysosomes where it is in turn activated. Adapter protein that enhances the efficiency of the MAP kinase cascade facilitating the activation of MAPK2.
Sequence similaritiesBelongs to the LAMTOR3 family.
ab32134 showing positive staining in Breast carcinoma tissue.
References for Anti-MAP2K1IP1 antibody [Y130] (ab32134)
This product has been referenced in:
Schiefermeier N et al. The late endosomal p14-MP1 (LAMTOR2/3) complex regulates focal adhesion dynamics during cell migration. J Cell Biol205:525-540 (2014).
Read more (PubMed: 24841562) »
Westerman BA et al. A genome-wide RNAi screen in mouse embryonic stem cells identifies Mp1 as a key mediator of differentiation. J Exp Med208:2675-89 (2011).
Read more (PubMed: 22143885) »