Anti-MAP3K12 antibody (ab37996)

Overview

  • Product name
  • Description
    Rabbit polyclonal to MAP3K12
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human MAP3K12 aa 828-859 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: Q12852

  • Positive control
    • WB: HL-60 and SH-SY5Y cell lysates and mouse kidney tissue lysate. IHC-P: Human breast carcinoma tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This antibody is purified through a protein A column, followed by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab37996 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 95 kDa.
IHC-P 1/50 - 1/100.

Target

  • Function
    May be an activator of the JNK/SAPK pathway. Phosphorylates beta-casein, histone 1 and myelin basic protein in vitro.
  • Tissue specificity
    Highly expressed in brain and kidney.
  • Sequence similarities
    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
    Contains 1 protein kinase domain.
  • Domain
    Interacts with MBIP through the leucine-zipper motif.
  • Post-translational
    modifications
    Autophosphorylated on Ser/Thr. Phosphorylated in cytosol under basal conditions and dephosphorylated when membrane-associated.
  • Cellular localization
    Cytoplasm. Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Dlk antibody
    • Dual leucine zipper bearing kinase antibody
    • Dual leucine zipper kinase DLK antibody
    • Leucine-zipper protein kinase antibody
    • M3K12_HUMAN antibody
    • Map3k12 antibody
    • MAPK-upstream kinase antibody
    • MEKK12 antibody
    • Mitogen-activated protein kinase kinase kinase 12 antibody
    • Mixed lineage kinase antibody
    • MUK antibody
    • Protein kinase MUK antibody
    • ZPK antibody
    • ZPKP1 antibody
    see all

Images

  • All lanes : Anti-MAP3K12 antibody (ab37996) at 1/1000 dilution

    Lane 1 : HL-60 cell lysate
    Lane 2 : SH-SY5Y cell lysate

    Lysates/proteins at 35 µg per lane.

    Secondary
    HRP-conjugated goat anti-rabbit IgG (H+L) at 1/10000 dilution
  • All lanes : Anti-MAP3K12 antibody (ab37996)

    Lane 1 : HL-60 cell lysate
    Lane 2 : Mouse kidney tissue lysate

    Secondary
    HRP-conjugated anti-rabbit IgG

    Observed band size : 95 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 18 kDa,23 kDa,30 kDa,44 kDa. We are unsure as to the identity of these extra bands.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling MAP3K12 with ab37996. A peroxidase-conjugated anti-rabbit IgG was used as the secondary antibody, followed by AEC staining.

References

ab37996 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (Spinal Cord)
Loading amount
20 µg
Specification
Spinal Cord
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Jan 11 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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