Anti-MAP3K12 antibody (ab37996)


  • Product nameAnti-MAP3K12 antibody
    See all MAP3K12 primary antibodies
  • Description
    Rabbit polyclonal to MAP3K12
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human MAP3K12 aa 828-859 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: Q12852

  • Positive control
    • WB: HL-60 and SH-SY5Y cell lysates and mouse kidney tissue lysate. IHC-P: Human breast carcinoma tissue.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThis antibody is purified through a protein A column, followed by peptide affinity purification.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab37996 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 95 kDa.
IHC-P 1/50 - 1/100.


  • FunctionMay be an activator of the JNK/SAPK pathway. Phosphorylates beta-casein, histone 1 and myelin basic protein in vitro.
  • Tissue specificityHighly expressed in brain and kidney.
  • Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
    Contains 1 protein kinase domain.
  • DomainInteracts with MBIP through the leucine-zipper motif.
  • Post-translational
    Autophosphorylated on Ser/Thr. Phosphorylated in cytosol under basal conditions and dephosphorylated when membrane-associated.
  • Cellular localizationCytoplasm. Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Dlk antibody
    • Dual leucine zipper bearing kinase antibody
    • Dual leucine zipper kinase DLK antibody
    • Leucine-zipper protein kinase antibody
    • M3K12_HUMAN antibody
    • Map3k12 antibody
    • MAPK-upstream kinase antibody
    • MEKK12 antibody
    • Mitogen-activated protein kinase kinase kinase 12 antibody
    • Mixed lineage kinase antibody
    • MUK antibody
    • Protein kinase MUK antibody
    • ZPK antibody
    • ZPKP1 antibody
    see all

Anti-MAP3K12 antibody images

  • All lanes : Anti-MAP3K12 antibody (ab37996) at 1/1000 dilution

    Lane 1 : HL-60 cell lysate
    Lane 2 : SH-SY5Y cell lysate

    Lysates/proteins at 35 µg per lane.

    HRP-conjugated goat anti-rabbit IgG (H+L) at 1/10000 dilution
  • All lanes : Anti-MAP3K12 antibody (ab37996)

    Lane 1 : HL-60 cell lysate
    Lane 2 : Mouse kidney tissue lysate

    HRP-conjugated anti-rabbit IgG

    Observed band size : 95 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 18 kDa,23 kDa,30 kDa,44 kDa. We are unsure as to the identity of these extra bands.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling MAP3K12 with ab37996. A peroxidase-conjugated anti-rabbit IgG was used as the secondary antibody, followed by AEC staining.

References for Anti-MAP3K12 antibody (ab37996)

ab37996 has not yet been referenced specifically in any publications.

Product Wall

Thank you for submitting your Abreview. I hope you have been notified that it has been published on our website. I wanted to follow up with you regarding your review as your rating of 'Inconclusive' indicated you may be unhappy with the performance of ...

Read More
Application Western blot
Sample Mouse Tissue lysate - whole (Spinal Cord)
Loading amount 20 µg
Specification Spinal Cord
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jan 11 2012