Recombinant
RabMAb

Anti-MAP3K12 binding inhibitory protein 1 antibody [EPR13952] (ab181040)

Overview

  • Product name
    Anti-MAP3K12 binding inhibitory protein 1 antibody [EPR13952]
    See all MAP3K12 binding inhibitory protein 1 primary antibodies
  • Description
    Rabbit monoclonal [EPR13952] to MAP3K12 binding inhibitory protein 1
  • Tested applications
    Suitable for: WB, ICC/IF, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human MAP3K12 binding inhibitory protein 1 aa 300 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: Q9NS73

  • Positive control
    • 293T, HepG2, HeLa and Caco-2 cell lysates. HepG2 cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 0.05% BSA, 40% Glycerol
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR13952
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab181040 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 39 kDa.
ICC/IF 1/100 - 1/250.
IP 1/10 - 1/100.

Target

  • Function
    Inhibits the MAP3K12 activity to induce the activation of the JNK/SAPK pathway. Component of the ATAC complex, a complex with histone acetyltransferase activity on histones H3 and H4.
  • Tissue specificity
    Ubiquitous. High expression seen in the heart and lung.
  • Cellular localization
    Nucleus. Cytoplasm. Shows a cytoplasmic localization when coexpressed with MAP3K12.
  • Information by UniProt
  • Database links
  • Alternative names
    • MAP3K12 binding inhibitory protein 1 antibody
    • MAP3K12-binding inhibitory protein 1 antibody
    • MAPK upstream kinase binding inhibitory protein antibody
    • MAPK upstream kinase-binding inhibitory protein antibody
    • Mbip antibody
    • MBIP1_HUMAN antibody
    • MUK binding inhibitory protein antibody
    • MUK-binding inhibitory protein antibody
    • OTTHUMP00000178844 antibody
    see all

Images

  • All lanes : Anti-MAP3K12 binding inhibitory protein 1 antibody [EPR13952] (ab181040) at 1/1000 dilution

    Lane 1 : 293T cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : Hela cell lysate
    Lane 4 : Caco-2 cell lysate

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 39 kDa
  • Immunofluorescent staining of HepG2 cells labeling MAP3K12 binding inhibitory protein 1 with ab181040 at 1/100 dilution.

  • Western blot analysis on immunoprecipitation pellet from (Lane 1) 293T cell lysate or (Lane 2) 1XPBS (negative control) using ab181040 at 1/10 dilution for IP, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

References

ab181040 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab181040.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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