Recombinant fragment Predicted to work with:
Recombinant fragment: LYHLQLKLKS NCITENPAGP YGQRTDKELI DWLRLQGADA KTIEKIVEEG YTLSDILNEI TKEDLRYLRL RGGLLCRLWS AVSQYRRAQE ASETKD, corresponding to amino acids 691-787 of Human MAP3K15(NP_001001671), with a 26kDa enzyme tag.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesELISA: Use at an assay dependent dilution.
WB: Use at a concentration of 1 - 5 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).
This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionMay function in a signal transduction pathway that is activated by various cell stresses and leads to apoptosis.
Tissue specificityIsoform 2 and isoform 3 are widely expressed. Isoform 2 highest levels are observed in fetal brain, and isoform 3 highest levels in pancreas, peripheral blood leukocytes, fetal brain and spleen.
Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily. Contains 1 protein kinase domain.