Overview

  • Product nameAnti-MAP3K15 antibody
    See all MAP3K15 primary antibodies
  • Description
    Rabbit polyclonal to MAP3K15
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within residues: LEQKTQELYH LQLKLKSNCI TENPAGPYGQ RTDKELIDWL RLQGADAKTI, corresponding to internal sequence amino acids 684-733 of Human MAP3K15 isoform 3 (NP_001001671)

  • Positive control
    • Human fetal intestine lysate.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab90038 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 89 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionMay function in a signal transduction pathway that is activated by various cell stresses and leads to apoptosis.
  • Tissue specificityIsoform 2 and isoform 3 are widely expressed. Isoform 2 highest levels are observed in fetal brain, and isoform 3 highest levels in pancreas, peripheral blood leukocytes, fetal brain and spleen.
  • Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
    Contains 1 protein kinase domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • Apoptosis signal-regulating kinase 3 antibody
    • ASK3 antibody
    • bA723P2.3 antibody
    • FLJ16518 antibody
    • M3K15_HUMAN antibody
    • Map3k15 antibody
    • MAPK/ERK kinase kinase 15 antibody
    • MEK kinase 15 antibody
    • MEKK 15 antibody
    • Mitogen activated protein kinase kinase kinase 15 antibody
    • Mitogen-activated protein kinase kinase kinase 15 antibody
    • OTTHUMP00000183504 antibody
    see all

Anti-MAP3K15 antibody images

  • Anti-MAP3K15 antibody (ab90038) at 1 µg/ml + Human fetal intestine lysate at 10 µg

    Secondary
    anti-Rabbit IgG HRP at 1/50000 dilution

    Predicted band size : 89 kDa
    Observed band size : 89 kDa
    Additional bands at : 40 kDa. We are unsure as to the identity of these extra bands.

References for Anti-MAP3K15 antibody (ab90038)

ab90038 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunoprecipitation
Sample Mouse Tissue lysate - whole (HepG2)
Total protein in input 1000 µg
Specification HepG2
Immuno-precipitation step Protein A/G
Username

Dr. Avi Shtevi

Verified customer

Submitted Dec 20 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"