Anti-MAP3K15 antibody - N-terminal (ab170686)

Overview

  • Product name
    Anti-MAP3K15 antibody - N-terminal
    See all MAP3K15 primary antibodies
  • Description
    Rabbit polyclonal to MAP3K15 - N-terminal
  • Tested applications
    Suitable for: Flow Cyt, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human MAP3K15 aa 314-342 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q6ZN16

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab170686 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/50. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
IHC-P 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500. Predicted molecular weight: 147 kDa.

Target

  • Function
    May function in a signal transduction pathway that is activated by various cell stresses and leads to apoptosis.
  • Tissue specificity
    Isoform 2 and isoform 3 are widely expressed. Isoform 2 highest levels are observed in fetal brain, and isoform 3 highest levels in pancreas, peripheral blood leukocytes, fetal brain and spleen.
  • Sequence similarities
    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
    Contains 1 protein kinase domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • Apoptosis signal-regulating kinase 3 antibody
    • ASK3 antibody
    • bA723P2.3 antibody
    • FLJ16518 antibody
    • M3K15_HUMAN antibody
    • Map3k15 antibody
    • MAPK/ERK kinase kinase 15 antibody
    • MEK kinase 15 antibody
    • MEKK 15 antibody
    • Mitogen activated protein kinase kinase kinase 15 antibody
    • Mitogen-activated protein kinase kinase kinase 15 antibody
    • OTTHUMP00000183504 antibody
    see all

Images

  • Flow cytometric analysis of Neuro-2a cells labeling MAP3K15 with ab170686 at  1/10 dilution (right histogram) compared to negative control cells (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue labeling MAP3K15 with ab170686 at 1/10 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.

  • Anti-MAP3K15 antibody - N-terminal (ab170686) at 1/100 dilution + Neuro-2a cell line lysate at 35 µg

    Predicted band size : 147 kDa

References

ab170686 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (Brain)
Permeabilization
Yes - 30min in citrate buffer @ 90oC
Specification
Brain
Blocking step
Normal Goat Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative
Paraformaldehyde
Username

Dr. Emma Schofield

Verified customer

Submitted Sep 15 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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