Recombinant
RabMAb

Anti-MAP3K4 antibody [EP551Y] (ab40784)

Overview

  • Product name
    Anti-MAP3K4 antibody [EP551Y]
    See all MAP3K4 primary antibodies
  • Description
    Rabbit monoclonal [EP551Y] to MAP3K4
  • Specificity
    This antibody is predicted to detect splice isoform 2 based on sequence analysis.
  • Tested applications
    Suitable for: IHC-P, ICC, Flow Cyt, WBmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human MAP3K4 aa 150-250.
    Database link: Q9Y6R4

  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    A trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Alternative versions available:

    Anti-MEK4 antibody (Alexa Fluor® 488) [EP551Y] (ab200611)

    Anti-MEK4 antibody (Alexa Fluor® 647) [EP551Y] (ab200522)

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab40784 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
ICC 1/100 - 1/250.
Flow Cyt 1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB 1/1000 - 1/5000. Detects a band of approximately 200 kDa (predicted molecular weight: 182 kDa).
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      Component of a protein kinase signal transduction cascade. Activates the CSBP2, P38 and JNK MAPK pathways, but not the ERK pathway. Specifically phosphorylates and activates MAP2K4 and MAP2K6.
    • Tissue specificity
      Expressed at high levels in heart, placenta, skeletal muscle and pancreas, and at lower levels in other tissues.
    • Sequence similarities
      Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
      Contains 1 protein kinase domain.
    • Cellular localization
      Cytoplasm > perinuclear region. Localized in perinuclear vesicular-like structures, probably Golgi-associated vesicles.
    • Information by UniProt
    • Database links
    • Alternative names
      • dJ473J16.1 (mitogen-activated protein kinase kinase kinase 4) antibody
      • FLJ42439 antibody
      • KIAA0213 antibody
      • M3K4_HUMAN antibody
      • MAP three kinase 1 antibody
      • MAP/ERK kinase kinase 4 antibody
      • MAP3K4 antibody
      • MAPK/ERK kinase kinase 4 antibody
      • MAPKKK4 antibody
      • MEK kinase 4 antibody
      • MEKK 4 antibody
      • MEKK4 antibody
      • Mitogen-activated protein kinase kinase kinase 4 antibody
      • MTK1 antibody
      • predicted protein of HQ0412 antibody
      • PRO0412 antibody
      • RP3-473J16.4 antibody
      • SSK2/SSK22 MAP kinase kinase kinase, yeast, homolog of antibody
      see all

    Images

    • Anti-MAP3K4 antibody [EP551Y] (ab40784) at 1/1000 dilution + 293 cell lysate. at 10 µg

      Predicted band size : 182 kDa
      Observed band size : 200 kDa (why is the actual band size different from the predicted?)
    • Immunohistochemical analysis of paraffin-embedded human ovary adenocarcinoma using ab40784.

    References

    ab40784 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab40784.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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