Overview

  • Product nameAnti-MAP3K8 antibody
    See all MAP3K8 primary antibodies
  • Description
    Rabbit polyclonal to MAP3K8
  • Tested applicationsSuitable for: IHC-P, ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthesized non-phosphopeptide derived from human MAP3K8 around the phosphorylation site of threonine 290 (R-G-TP-E-I).

  • Positive control
    • Human brain tissue; extracts from 293 cells treated with LPS

Properties

Applications

Our Abpromise guarantee covers the use of ab51208 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
ELISA 1/40000.
WB 1/300 - 1/1000. Predicted molecular weight: 53 kDa.

Target

  • FunctionRequired for TLR4 activation of the MEK/ERK pathway. Able to activate NF-kappa-B 1 by stimulating proteasome-mediated proteolysis of NF-kappa-B 1/p105. Plays a role in the cell cycle. The longer form has some transforming activity, although it is much weaker than the activated cot oncoprotein.
  • Tissue specificityExpressed in several normal tissues and human tumor-derived cell lines.
  • Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
    Contains 1 protein kinase domain.
  • Developmental stageIsoform 1 is activated specifically during the S and G2/M phases of the cell cycle.
  • Post-translational
    modifications
    Autophosphorylated. Isoform 1 undergoes phosphorylation mainly on Ser residues, and isoform 2 on both Ser and Thr residues.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • c COT antibody
    • Cancer Osaka thyroid oncogene antibody
    • CCOT antibody
    • COT antibody
    • COT proto oncogene serine/threonine protein kinase antibody
    • EST antibody
    • ESTF antibody
    • Ewing sarcoma transformant antibody
    • FLJ10486 antibody
    • M3K8_HUMAN antibody
    • MAP3K 8 antibody
    • MAP3K8 antibody
    • MEKK8 antibody
    • Mitogen activated protein kinase kinase kinase 8 antibody
    • Mitogen-activated protein kinase kinase kinase 8 antibody
    • Proto oncogene cCot antibody
    • Proto-oncogene c-Cot antibody
    • Serine/threonine protein kinase cot antibody
    • Serine/threonine-protein kinase cot antibody
    • TPL 2 antibody
    • TPL-2 antibody
    • TPL2 antibody
    • Tumor progression locus 2 antibody
    see all

Anti-MAP3K8 antibody images

  • Immunohistochemical analysis of paraffin-embedded human brain tissue using ab51208, with and without immunising peptide.
  • All lanes : Anti-MAP3K8 antibody (ab51208)

    Lane 1 : 293 cells treated with LPS (100ng/ml, 30min) with no immunizing peptide
    Lane 2 : 293 cells treated with LPS (100ng/ml, 30min) with immunizing peptide


    Predicted band size : 53 kDa
    Observed band size : ~60 kDa (why is the actual band size different from the predicted?)

References for Anti-MAP3K8 antibody (ab51208)

ab51208 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your response and for providing some details.

It is likely that the protein is aggregating during cell lysis which could explain the higher molecular weight. I have attached our aggregation treatment protocol you may wish to con...

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