Anti-MAP3K8 (phospho S400) antibody (ab111367)


  • Product nameAnti-MAP3K8 (phospho S400) antibody
    See all MAP3K8 primary antibodies
  • Description
    Rabbit polyclonal to MAP3K8 (phospho S400)
  • Specificityab111367 detects endogenous levels of MAP3K8 only when phosphorylated at serine 400.
  • Tested applicationsSuitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic phosphopeptide derived from Human MAP3K8 around the phosphorylation site of serine 400 (C-Q-SP-L-D).

  • Positive control
    • Human brain tissue.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferpH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 49.1% PBS, 50% Glycerol, 0.88% Sodium chloride
    Note: PBS (without Mg2+ and Ca2+)
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab111367 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab111367 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.


  • FunctionRequired for TLR4 activation of the MEK/ERK pathway. Able to activate NF-kappa-B 1 by stimulating proteasome-mediated proteolysis of NF-kappa-B 1/p105. Plays a role in the cell cycle. The longer form has some transforming activity, although it is much weaker than the activated cot oncoprotein.
  • Tissue specificityExpressed in several normal tissues and human tumor-derived cell lines.
  • Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
    Contains 1 protein kinase domain.
  • Developmental stageIsoform 1 is activated specifically during the S and G2/M phases of the cell cycle.
  • Post-translational
    Autophosphorylated. Isoform 1 undergoes phosphorylation mainly on Ser residues, and isoform 2 on both Ser and Thr residues.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • c COT antibody
    • Cancer Osaka thyroid oncogene antibody
    • CCOT antibody
    • COT antibody
    • COT proto oncogene serine/threonine protein kinase antibody
    • EST antibody
    • ESTF antibody
    • Ewing sarcoma transformant antibody
    • FLJ10486 antibody
    • M3K8_HUMAN antibody
    • MAP3K 8 antibody
    • MAP3K8 antibody
    • MEKK8 antibody
    • Mitogen activated protein kinase kinase kinase 8 antibody
    • Mitogen-activated protein kinase kinase kinase 8 antibody
    • Proto oncogene cCot antibody
    • Proto-oncogene c-Cot antibody
    • Serine/threonine protein kinase cot antibody
    • Serine/threonine-protein kinase cot antibody
    • TPL 2 antibody
    • TPL-2 antibody
    • TPL2 antibody
    • Tumor progression locus 2 antibody
    see all

Anti-MAP3K8 (phospho S400) antibody images

  • ab111367 at 1/50 dilution staining MAP3K8 in paraffin-embedded Human Brain tissue by Immunohistochemistry. The image on the right is treated with the synthesized phoshpopeptide.

References for Anti-MAP3K8 (phospho S400) antibody (ab111367)

ab111367 has not yet been referenced specifically in any publications.

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